α-galactosidase (gene Agas2) from a Hermetia illucens gut metagenomic library

Enzyme Description

Species

Na (Hermetia illucens gut metagenomic library)

Extremophile

No but "halotolerant" enzyme

EC Number

3.2.1.22 ↗

Sequence

Length: 668 amino acids

MRNTNKSYILLLILAIICCRTSVSAQKQFLVQSPDNELKVNISVGNTIEYSVSHNGDLMIANSPISMSLTNGKSFGINPRLAGLSSKTLNDTITASVYKRKEIADTYNELILRFKEGFSLVFRAYNDGIAYRFVSNLKKPFEVKSEEVNFNFPTDQKAYIPYVQIIKEPIEKQFYNSFENTYQHINISQWDKKRLAFLPLVVEGVNGKKVCITESDLTNYPGMYLYNGNGSTTLSGVFAPYPKDIKQGGHNMLQGEVQSAEPYIARYNGATNFPWRIIIVSENDKELADNDMVYKLATPNKLADASWIKPGKVAWDWWNDWNLYDVDFRSGINNETYKYYIDFASQHGIEYVILDEGWAVNLQADLFQVVPEIDLKELVDYANKKNVGLILWAGYYAFNKDIEGICKHYSEMGIKGFKVDFMDRDDQPMVDFHYRAAEIAAKYHMMLDYHGTYKPTGLQRTFPNVINFEGVHGLEQMKWSPESVDQVTYDVTIPFIRMVAGPLDYTQGAMRNASKGNYRPVNSEPMSQGTRCRQLAEYIIFESPLNMLCDNPSNYMREKECTEFIANVPTVWDNTIALNGKIGEYITIAREKDDVWYVGSLTNWDARTLDVDLSFLGEGSFKAEVFKDGANADRSGHDYKKEVIDIPSDRKLSIPMASGGGYVMKIYK

Chang-Muk Lee et al. (2018) — Isolation and characterization of a halotolerant and protease-resistant α-galactosidase from the gut metagenome of Hermetia illucens

Journal of Biotechnology · doi:10.1016/j.jbiotec.2018.05.003 ↗ · Stability - Incubation

8 measurements ▼

Database ID

UniProt: A0A1W6DZM5 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3) pLysS

Experimental Data (8 measurements)

8 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100	97	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	Incubation: 7, Assay: 7	Incubation: 30°C, Assay: 40°C	1 mM p-Nitrophenyl α-D-galactopyranoside	D-Galactose , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100	96	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	Incubation: 7, Assay: 7	Incubation: 30°C, Assay: 40°C	1 mM p-Nitrophenyl α-D-galactopyranoside	D-Galactose , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	10%	100	98	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	Incubation: 7, Assay: 7	Incubation: 30°C, Assay: 40°C	1 mM p-Nitrophenyl α-D-galactopyranoside	D-Galactose , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	10%	100	91	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	Incubation: 7, Assay: 7	Incubation: 30°C, Assay: 40°C	1 mM p-Nitrophenyl α-D-galactopyranoside	D-Galactose , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	10%	100	91	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	Incubation: 7, Assay: 7	Incubation: 30°C, Assay: 40°C	1 mM p-Nitrophenyl α-D-galactopyranoside	D-Galactose , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	10%	100	70	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	Incubation: 7, Assay: 7	Incubation: 30°C, Assay: 40°C	1 mM p-Nitrophenyl α-D-galactopyranoside	D-Galactose , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	10%	100	61	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	Incubation: 7, Assay: 7	Incubation: 30°C, Assay: 40°C	1 mM p-Nitrophenyl α-D-galactopyranoside	D-Galactose , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Butanol	10%	100	12	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	Incubation: 7, Assay: 7	Incubation: 30°C, Assay: 40°C	1 mM p-Nitrophenyl α-D-galactopyranoside	D-Galactose , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100	97	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	Incubation: 7, Assay: 7	Incubation: 30°C, Assay: 40°C	1 mM p-Nitrophenyl α-D-galactopyranoside	D-Galactose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100	96	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	Incubation: 7, Assay: 7	Incubation: 30°C, Assay: 40°C	1 mM p-Nitrophenyl α-D-galactopyranoside	D-Galactose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	10%	100	98	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	Incubation: 7, Assay: 7	Incubation: 30°C, Assay: 40°C	1 mM p-Nitrophenyl α-D-galactopyranoside	D-Galactose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	10%	100	91	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	Incubation: 7, Assay: 7	Incubation: 30°C, Assay: 40°C	1 mM p-Nitrophenyl α-D-galactopyranoside	D-Galactose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	10%	100	91	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	Incubation: 7, Assay: 7	Incubation: 30°C, Assay: 40°C	1 mM p-Nitrophenyl α-D-galactopyranoside	D-Galactose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	10%	100	70	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	Incubation: 7, Assay: 7	Incubation: 30°C, Assay: 40°C	1 mM p-Nitrophenyl α-D-galactopyranoside	D-Galactose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	10%	100	61	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	Incubation: 7, Assay: 7	Incubation: 30°C, Assay: 40°C	1 mM p-Nitrophenyl α-D-galactopyranoside	D-Galactose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Butanol	10%	100	12	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	Incubation: 7, Assay: 7	Incubation: 30°C, Assay: 40°C	1 mM p-Nitrophenyl α-D-galactopyranoside	D-Galactose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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