Endoglucanase (gene CelCM3) from a camel rumen metagenome
Enzyme Description
Species
Na (camel rumen metagenomic library
Extremophile
No
but"cold-adapted" enzyme
EC Number
Sequence
MFLLFSSCGGGDSTNAGEESPKDDKEKKEEMVSALDITKKLGWGWNLGNHFDTSTGTDNKPFEWGYWDNATPTQTLYTRLKSAGVSTVRIAITWDNNQSAPNWTIKSSYMAEVKQNVEWAEAAGLNVILNMHHDGYWLKIKEAANDATVNTQIKERIQKTWKQIAEAFKDKGDFLFFETFNEIHDGGWGWGDNRKDGGKQYRTLNEWNQLAVNTIRATGSKNATRWIGIPSYVASPQCALESTFVLPTDPANRLMVSVHYYDPSNFTLTPENKDGKTEWGHTATEGNYVVGNNEEHVTEVFQKLYQKFIAKNIPVYIGEYGCVMQNTERGNLFSKYYLEYVCRAAHTYHLPLIIWDNNQPGSGNEHHAYFNHSNGNYQDGMQSLVQTMIKAATSDDSSYTLQSIKNNAPR
Kamran Khalili Ghadikolaei et al. (2018)
β A cold-adapted endoglucanase from camel rumen with high catalytic activity at moderate and low temperatures: an anomaly of truly cold-adapted evolution in a mesophilic environment
Extremophiles
Β· doi:10.1007/s00792-018-0999-6 β
Β· Activity - Classical
▼
Database ID
UniProt: A0A1W5YNV1 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (20 measurements)
20 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | n-Hexane | 10% | 100.0 | 62.8 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Cyclohexane | 20% | 100.0 | 69.5 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Cyclohexane | 10% | 100.0 | 73.8 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Benzene | 20% | 100.0 | 43.6 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Benzene | 10% | 100.0 | 44.5 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Chloroform | 20% | 100.0 | 21.8 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Chloroform | 10% | 100.0 | 56.7 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Toluene | 20% | 100.0 | 56.9 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Toluene | 10% | 100.0 | 62.0 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | n-Hexane | 20% | 100.0 | 69.5 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Isopropanol | 10% | 100.0 | 27.5 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Acetone | 20% | 100.0 | 53.5 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Acetone | 10% | 100.0 | 56.9 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Isobutanol | 20% | 100.0 | 20.4 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Isobutanol | 10% | 100.0 | 22.5 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Ethanol | 20% | 100.0 | 25.7 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Ethanol | 10% | 100.0 | 32.3 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Methanol | 20% | 100.0 | 26.8 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Methanol | 10% | 100.0 | 31.6 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Isopropanol | 20% | 100.0 | 25.2 | % | Citrate buffer | 5 | 30Β°C | 0.9% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.