N-terminal truncated lipase from Bacillus sphaericus 205y

Enzyme Description

Species
Bacillus sphaericus 205y β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 300 amino acids
QPISTMKSYAKSENVSVALSSHFFYNQNISTKPSVDVVYGKTTDGIELKLDVWPAKKKSEDVLTPVIVQVHGGGWVSGDKGQVQDWNQWMNDQGYTVFDVQYRMPPVAGWKDEVGDVKSAIGWIVQHADTYKIDPNRIILMGESAGGNLAMLAAYSLGDKHLPPSTDVPDVPIKAVINMYGPSDMTAFYKNNPSKRYVQDVLDQYIGGSPSDYPARYKKLSPISYIQEHTPPTIMFLGTGDRIVPVEQANVLDDKLTTSGVAHELYLLPKVDHGFDANPGSLSTQFAKEKVKAFLQKYNK
Malihe Masomian et al. (2017) β€” Impact of signal peptide and transmembrane segments on expression and biochemical properties of a lipase from Bacillus sphaericus 205y
Journal of Biotechnology  Β· doi:10.1016/j.jbiotec.2017.10.014 β†—  Β· Stability - Incubation
11 measurements ▼
Database ID
UniProt: Q8VQP2 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number (97 first residues removed from UniProt sequence removed )
Protein Source
Recombinant, host bacterium Escherichia coli TOP10

Experimental Data (11 measurements)

11 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 75% 100.0 132.4 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 60Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase Methanol 75% 100.0 142.5 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 60Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase Ethanol 75% 100.0 138.0 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 60Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase Acetonitrile 75% 100.0 140.5 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 60Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase 1-Butanol 75% 100.0 12.5 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 60Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase Toluene 75% 100.0 23.0 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 60Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase p-Xylene 75% 100.0 46.0 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 60Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase 1-Decanol 75% 100.0 41.0 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 60Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase n-Decane 75% 100.0 121.5 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 60Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase n-Dodecane 75% 100.0 69.0 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 60Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase n-Hexadecane 75% 100.0 130.0 % Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer Incubation: 8, Assay: 8 Incubation: 37Β°C, Assay: 60Β°C 35% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm, Assay: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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