Esterase (gene e25) from Altererythrobacter epoxidivorans CGMCC 1.7731T
Enzyme Description
Extremophile
No
but "alkaline" enzyme
EC Number
Sequence
MADNQPYVREDVKAFLTLLEAAGGPALADMTLEEARQSYVALHGMADAPARDLAVIRNLCCPGPAGDIPLRLYDARESRDPSPVIMFYHGGGFVIGDLDTHHNLCTEIAHQMDLPVVAVDYRRAPENPFPAAIEDCEAAARWVAGSPEELGRKATGIVNIGDSAGGNATIVVTQQLAKNAANVPVVLQVPIFPLATDAIGSHSLDEFAEGYILTKAAILFFDAAYVPDRKDPRAMPILGQHEGTPPTVVATASLDPIRDSGRDYAAALSHAGVDHVFLEVSGGTHSFTNLRQAIPSYQGELERVFAAMKMFLGQN
Zhen Rong et al. (2018)
β Characterization of a novel alkaline esterase from Altererythrobacter epoxidivorans CGMCC 1.7731T
Preparative Biochemistry & Biotechnology
Β· doi:10.1080/10826068.2017.1387559 β
Β· Activity - Classical
▼
Database ID
UniParc: UPI0007856974 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (8 measurements)
8 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetone | 1% | 100.0 | 45.7 | % | 100 mM CHES-NaOH buffer | 9 | 45Β°C | 1 mM p-Nitrophenyl hexanoate | caproic acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetonitrile | 1% | 100.0 | 52.0 | % | 100 mM CHES-NaOH buffer | 9 | 45Β°C | 1 mM p-Nitrophenyl hexanoate | caproic acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 1% | 100.0 | 38.9 | % | 100 mM CHES-NaOH buffer | 9 | 45Β°C | 1 mM p-Nitrophenyl hexanoate | caproic acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 1% | 100.0 | 27.5 | % | 100 mM CHES-NaOH buffer | 9 | 45Β°C | 1 mM p-Nitrophenyl hexanoate | caproic acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 1% | 100.0 | 78.4 | % | 100 mM CHES-NaOH buffer | 9 | 45Β°C | 1 mM p-Nitrophenyl hexanoate | caproic acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Glycerol | 1% | 100.0 | 106.1 | % | 100 mM CHES-NaOH buffer | 9 | 45Β°C | 1 mM p-Nitrophenyl hexanoate | caproic acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Isopropanol | 1% | 100.0 | 32.1 | % | 100 mM CHES-NaOH buffer | 9 | 45Β°C | 1 mM p-Nitrophenyl hexanoate | caproic acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 1% | 100.0 | 69.9 | % | 100 mM CHES-NaOH buffer | 9 | 45Β°C | 1 mM p-Nitrophenyl hexanoate | caproic acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.