Lipase (gene PleoLip369) from Pleurotus ostreatus

Enzyme Description

Species
Pleurotus ostreatus β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 609 amino acids
MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLDKRGNTTLYGRNLSDSGLEFYGGIPYAEPPLGDLRLRPPVPKALDVPSFDASDFGLMCYQRDLPAEVMSEDCLTINVLRPAGISSDASLPVMAWVHGGGFDQGSASEYNGTAIVAQSVARGTPVIYVNFNYRLGPLGFPQGREAAEKRALNVGLRDMVLALNWIQDNIGVFGGDKAKVTVFGESAGAIALGTLMLGDTLDGLARAAIFQSGSAASTISVDTLDREADWHHFVTAIPACSMTAWTRDTFSCIRAADTSSLLPAVVAPLALSKQIFPWDNTIDGPGGFLPDLPSRLWERGLFAKIPFISGNNLDEGTLLTHPWVNSTEMLRETLIANYTPALMGERALNESVERLLELYPDVPALGSPFRTGNETFGLSSHFKRGCAILGDTIFHAQRRKFSAVANRFGVKNWGYLFSDPPTTGPAFQGVAHLAELPYIFGTIDTPSYAKELSSLMIDYWVSFATSLDPNDGKGLRRPVWPELTRRNQVLIEFIGNNTGVIPDNYRAEQIDFIMGNLPVFQARHHHHHH
Alessandra Piscitelli et al. (2017) β€” New lipases by mining of Pleurotus ostreatus genome
PLOS ONE  Β· doi:10.1371/journal.pone.0185377 β†—  Β· Stability - Incubation
14 measurements ▼
Database ID
UniProt: A0A8H7A800 β†—
Sequence Annotation
Explicit - Provided (first 22 residues from UniProt sequence replaced by the first 85 residues from the explicit sequence here, 6 terminal residues added as His-tag)
Protein Source
Recombinant, host yeast Pichia pastoris

Experimental Data (14 measurements)

14 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 10% 100 34 % Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2% isopropanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°c 0.2 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Glycerol 10% 100 55 % Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2% isopropanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°c 0.2 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 10% 100 20 % Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2% isopropanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°c 0.2 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 10% 100 40 % Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2% isopropanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°c 0.2 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methyl Acetate 10% 100 0 % Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2% isopropanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°c 0.2 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase tert-Butanol 10% 100 62 % Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2% isopropanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°c 0.2 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethyl Acetate 10% 100 45 % Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2% isopropanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°c 0.2 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 20% 100 0 % Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2% isopropanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°c 0.2 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Glycerol 20% 100 52 % Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2% isopropanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°c 0.2 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 20% 100 0 % Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2% isopropanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°c 0.2 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 20% 100 0 % Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2% isopropanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°c 0.2 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methyl Acetate 20% 100 0 % Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2% isopropanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°c 0.2 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase tert-Butanol 20% 100 35 % Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2% isopropanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°c 0.2 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethyl Acetate 20% 100 22 % Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2% isopropanol Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°c 0.2 mM p-Nitrophenyl decanoate Decanoic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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