Ξ±-amylase from Catenovulum sp. X3

Enzyme Description

Species
Catenovulum sp. X3 β†—
Extremophile
No but " cold-adaptive", "organic solvent-tolerant" and "alkaline" enzyme
EC Number
3.2.1.1 β†—

Sequence

Length: 664 amino acids
MNKKLTQVIKGVLAATVLSTTYSNTASADVMLHAFNWQYDSVTERADEIAALGYKSVLVSPPYLSNPNSSWWARYQPLDYRILLGPLGNKQDFIDMVNALEARGVNLYVDVIFNHMANMGMAKGQEQYPGFNVNTPEYNANKLYGDLSGGLFGPGDFHDGANAKCISNWGDWFDSTWNRLCGTAPDIGLPDLRGEATDGSGGWIRQNQIAYLEALKALGVDGFRIDAVKHMKWEHLNWVFNSDVLDGIHVFSEIISHSGADDSTYLQPYLDNTDVRMGAYDFRLFNEIDHAMDVNGSLSSLAQPNTLPNDRAVTFVITHDMPQNDGFNSFLFSEEDEKLAYAYILGRDGGVPLVYTDNNETPKFNSRWVDAYKNGDIASMITFHNSVQGTPMQVLSADGCSIYFRRGESGLVGINKCGNAVTKTLNTTGLNIGTYTDQLTGSTLNISGASQGVTIPARSARMWLGGGTPPPPAVWYFSGTANTWGADQMTQEGSVFTITKAFGTGDANGGPRFKVRDSNTNWNNNCPTADFAVEANKTYKITFDPAVANCAGLSVQEEVIIQPGGSCPITFSCANGNTNMGDSVYVVGSATEIGSWNAADAVKLNPTNYPTWEGTINIPKNTQVSWKCIIRQEGSPFTVRQWEQDPNNNFNTGNCDVTQAQGSF
Yi-Rui Wu et al. (2017) β€” Catalytic hydrolysis of starch for biohydrogen production by using a newly identified amylase from a marine bacterium Catenovulum sp. X3
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2017.06.084 β†—  Β· Stability - Incubation
14 measurements ▼
Database ID
UniProt: A0A218MJE6 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (14 measurements)

14 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 20% 100.0 123.0 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 7.9 Incubation: 25Β°C, Assay: 35Β°C 0.1% (w/v) Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100.0 85.7 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 7.9 Incubation: 25Β°C, Assay: 35Β°C 0.1% (w/v) Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 20% 100.0 124.3 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 7.9 Incubation: 25Β°C, Assay: 35Β°C 0.1% (w/v) Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 50% 100.0 102.0 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 7.9 Incubation: 25Β°C, Assay: 35Β°C 0.1% (w/v) Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetone 20% 100.0 136.9 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 7.9 Incubation: 25Β°C, Assay: 35Β°C 0.1% (w/v) Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetone 50% 100.0 106.7 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 7.9 Incubation: 25Β°C, Assay: 35Β°C 0.1% (w/v) Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Chloroform 20% 100.0 141.5 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 7.9 Incubation: 25Β°C, Assay: 35Β°C 0.1% (w/v) Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Chloroform 50% 100.0 136.5 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 7.9 Incubation: 25Β°C, Assay: 35Β°C 0.1% (w/v) Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Benzene 20% 100.0 131.9 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 7.9 Incubation: 25Β°C, Assay: 35Β°C 0.1% (w/v) Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Benzene 50% 100.0 131.9 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 7.9 Incubation: 25Β°C, Assay: 35Β°C 0.1% (w/v) Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase n-Hexane 20% 100.0 155.4 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 7.9 Incubation: 25Β°C, Assay: 35Β°C 0.1% (w/v) Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase n-Hexane 50% 100.0 144.5 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 7.9 Incubation: 25Β°C, Assay: 35Β°C 0.1% (w/v) Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethyl Acetate 20% 100.0 107.1 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 7.9 Incubation: 25Β°C, Assay: 35Β°C 0.1% (w/v) Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethyl Acetate 50% 100.0 95.7 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 7.9 Incubation: 25Β°C, Assay: 35Β°C 0.1% (w/v) Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about control

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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