Peroxidase from Trachycarpus fortunei (chinese windmill palm)

Enzyme Description

Species

Trachycarpus fortunei (chinese windmill palm) ↗

Extremophile

EC Number

1.11.1.7 ↗

Sequence

Length: 344 amino acids

MSRPVKLFFLAFLALLAAVHGDLQIGFYNQSCPSAESLVQQAVAAAFANNSGIAPGLIRMHFHDCFVRGCDASVLLDSTANNTAEKDAAPNNPSLRGFEVIAAAKSAVEAACPKTVSCADILAFAARDSAALAGNITYQVPSGRRDGNVSLASEALTNIPAPTFNATQLINSFAGKNLTADEMVTLSGAHSIGVSHCFSFLNRIYNFSNTSQVDPTLSSSYADLLRTKCPSNSTRFTPITVSLDIITPTVLDNRYYTGVQLTLGLLTSDQALVTEANLSAAVKNNADNLTAWVAKFAQAIVKMGQIQVLTGTQGEIRTNCSVVNSASLGDIVMASGHLTEVATS

Boting Wen et al. (2017) — Expression and Characterization of Windmill Palm Tree ( Trachycarpus fortunei ) Peroxidase by Pichia pastoris

Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.7b00318 ↗ · Activity - Classical

7 measurements ▼

Database ID

UniProt: A0A0A0Y4H8 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host yeast Pichia pastoris

Experimental Data (7 measurements)

7 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100	94	%	10 mM Tris-HCl	7	Room temperature	0.1 mM ABTS, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	Cofactor : heme	—	Classical aqueous control (in %) \| Uncertainty about pH, deduced from buffer nature
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100	92	%	10 mM Tris-HCl	7	Room temperature	0.1 mM ABTS, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	Cofactor : heme	—	Classical aqueous control (in %) \| Uncertainty about pH, deduced from buffer nature
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	20%	100	96	%	10 mM Tris-HCl	7	Room temperature	0.1 mM ABTS, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	Cofactor : heme	—	Classical aqueous control (in %) \| Uncertainty about pH, deduced from buffer nature
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 405 nm) in the presence of organic solvent	Ethylene Glycol	20%	100	93	%	10 mM Tris-HCl	7	Room temperature	0.1 mM ABTS, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	Cofactor : heme	—	Classical aqueous control (in %) \| Uncertainty about pH, deduced from buffer nature
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	66	%	10 mM Tris-HCl	7	Room temperature	0.1 mM ABTS, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	Cofactor : heme	—	Classical aqueous control (in %) \| Uncertainty about pH, deduced from buffer nature
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	81	%	10 mM Tris-HCl	7	Room temperature	0.1 mM ABTS, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	Cofactor : heme	—	Classical aqueous control (in %) \| Uncertainty about pH, deduced from buffer nature
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100	99	%	10 mM Tris-HCl	7	Room temperature	0.1 mM ABTS, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation , H2O	Cofactor : heme	—	Classical aqueous control (in %) \| Uncertainty about pH, deduced from buffer nature

Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100	94	%	10 mM Tris-HCl	7	Room temperature	0.1 mM ABTS, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	Cofactor : heme	—	Classical aqueous control (in %) \| Uncertainty about pH, deduced from buffer nature
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100	92	%	10 mM Tris-HCl	7	Room temperature	0.1 mM ABTS, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	Cofactor : heme	—	Classical aqueous control (in %) \| Uncertainty about pH, deduced from buffer nature
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	20%	100	96	%	10 mM Tris-HCl	7	Room temperature	0.1 mM ABTS, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	Cofactor : heme	—	Classical aqueous control (in %) \| Uncertainty about pH, deduced from buffer nature
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 405 nm) in the presence of organic solvent	Ethylene Glycol	20%	100	93	%	10 mM Tris-HCl	7	Room temperature	0.1 mM ABTS, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	Cofactor : heme	—	Classical aqueous control (in %) \| Uncertainty about pH, deduced from buffer nature
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	66	%	10 mM Tris-HCl	7	Room temperature	0.1 mM ABTS, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	Cofactor : heme	—	Classical aqueous control (in %) \| Uncertainty about pH, deduced from buffer nature
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	81	%	10 mM Tris-HCl	7	Room temperature	0.1 mM ABTS, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	Cofactor : heme	—	Classical aqueous control (in %) \| Uncertainty about pH, deduced from buffer nature
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100	99	%	10 mM Tris-HCl	7	Room temperature	0.1 mM ABTS, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation, H2O	Cofactor : heme	—	Classical aqueous control (in %) \| Uncertainty about pH, deduced from buffer nature

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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