Laccase (gene lacM) from an acidic bog soil metagenomic library

Enzyme Description

Species

Na (acidic bog soil metagenomic library)

Extremophile

No but "thermostable" enzyme

EC Number

1.10.3.2 ↗

Sequence

Length: 452 amino acids

MNRREFLLTAGAVATGAGRSLVVPARLEAAAKADHTIRIAPVSFEIAPGKTIQTTGYNGKVPGPLLRMKEGKPVTVDVFNDTSSPEFLHWHGMYLSTKADGAEEEGSPVIPPHGHLRVSFTPKPAGTRWYHTHSMAMDDVTKGAYSGQFGFLYVEPKTDPGHYDQEIFLASRHWEPKIIHRGEPNNDWNLDYASATLGERSLGHGEPIRVKKGQRVLFRLLNANATRDVNLALAGHQFKVVALDGNPVPNPANVDILQLVVAERVDAVVEMNNPGVWILGSPRDDERARGLGIVVEYENEKGEPQWKTLPNTPFDYTLFGNPPAANPQPVEGSFDLSFHMLPDEGHAFNKWVVNDKVWPNTDPLMVKAGKRYRLVMHSGHEDSHPVHLHRHSFEIVKVGDKRTSGIFKDVVRVQRDNSTEVEFVADNPGNSLLHCHMQQHMDYGFKTLVKYS

Luka Ausec et al. (2017) — The first acidobacterial laccase-like multicopper oxidase revealed by metagenomics shows high salt and thermo-tolerance

Applied Microbiology and Biotechnology · doi:10.1007/s00253-017-8345-y ↗ · Activity - Classical

8 measurements ▼

Database ID

UniParc: UPI000BC0B903 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3) pLysS

Experimental Data (8 measurements)

8 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	1%	100	117	%	Multi-component buffer : 10 mM Trizma base, 15 mM sodium car-bonate, 15 mM phosphoric acid, 250 mM potassium chloride	4	25°C	1 mM ABTS	ABTS radical cation , H2O	50 µM CuSO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	10%	100	95	%	Multi-component buffer : 10 mM Trizma base, 15 mM sodium car-bonate, 15 mM phosphoric acid, 250 mM potassium chloride	4	25°C	1 mM ABTS	ABTS radical cation , H2O	50 µM CuSO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	1%	100	137	%	Multi-component buffer : 10 mM Trizma base, 15 mM sodium car-bonate, 15 mM phosphoric acid, 250 mM potassium chloride	4	25°C	1 mM ABTS	ABTS radical cation , H2O	50 µM CuSO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	91	%	Multi-component buffer : 10 mM Trizma base, 15 mM sodium car-bonate, 15 mM phosphoric acid, 250 mM potassium chloride	4	25°C	1 mM ABTS	ABTS radical cation , H2O	50 µM CuSO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	1%	100	143	%	Multi-component buffer : 10 mM Trizma base, 15 mM sodium car-bonate, 15 mM phosphoric acid, 250 mM potassium chloride	4	25°C	1 mM ABTS	ABTS radical cation , H2O	50 µM CuSO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	10%	100	154	%	Multi-component buffer : 10 mM Trizma base, 15 mM sodium car-bonate, 15 mM phosphoric acid, 250 mM potassium chloride	4	25°C	1 mM ABTS	ABTS radical cation , H2O	50 µM CuSO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100	96	%	Multi-component buffer : 10 mM Trizma base, 15 mM sodium car-bonate, 15 mM phosphoric acid, 250 mM potassium chloride	4	25°C	1 mM ABTS	ABTS radical cation , H2O	50 µM CuSO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	48	%	Multi-component buffer : 10 mM Trizma base, 15 mM sodium car-bonate, 15 mM phosphoric acid, 250 mM potassium chloride	4	25°C	1 mM ABTS	ABTS radical cation , H2O	50 µM CuSO₄	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	1%	100	117	%	Multi-component buffer : 10 mM Trizma base, 15 mM sodium car-bonate, 15 mM phosphoric acid, 250 mM potassium chloride	4	25°C	1 mM ABTS	ABTS radical cation, H2O	50 µM CuSO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	10%	100	95	%	Multi-component buffer : 10 mM Trizma base, 15 mM sodium car-bonate, 15 mM phosphoric acid, 250 mM potassium chloride	4	25°C	1 mM ABTS	ABTS radical cation, H2O	50 µM CuSO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	1%	100	137	%	Multi-component buffer : 10 mM Trizma base, 15 mM sodium car-bonate, 15 mM phosphoric acid, 250 mM potassium chloride	4	25°C	1 mM ABTS	ABTS radical cation, H2O	50 µM CuSO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	91	%	Multi-component buffer : 10 mM Trizma base, 15 mM sodium car-bonate, 15 mM phosphoric acid, 250 mM potassium chloride	4	25°C	1 mM ABTS	ABTS radical cation, H2O	50 µM CuSO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	1%	100	143	%	Multi-component buffer : 10 mM Trizma base, 15 mM sodium car-bonate, 15 mM phosphoric acid, 250 mM potassium chloride	4	25°C	1 mM ABTS	ABTS radical cation, H2O	50 µM CuSO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	10%	100	154	%	Multi-component buffer : 10 mM Trizma base, 15 mM sodium car-bonate, 15 mM phosphoric acid, 250 mM potassium chloride	4	25°C	1 mM ABTS	ABTS radical cation, H2O	50 µM CuSO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100	96	%	Multi-component buffer : 10 mM Trizma base, 15 mM sodium car-bonate, 15 mM phosphoric acid, 250 mM potassium chloride	4	25°C	1 mM ABTS	ABTS radical cation, H2O	50 µM CuSO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	48	%	Multi-component buffer : 10 mM Trizma base, 15 mM sodium car-bonate, 15 mM phosphoric acid, 250 mM potassium chloride	4	25°C	1 mM ABTS	ABTS radical cation, H2O	50 µM CuSO₄	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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