Lipase from Bacillus sp. PU1

Enzyme Description

Species
Bacillus sp. PU1 β†—
Extremophile
No but "highly alkaline" enzyme
EC Number
3.1.1.3 β†—

Sequence

Length: 212 amino acids
MKFVKRRIIALVTILMLSVTSLFALQPSAKAAEHNPVVMVHGIGGASFNFAGIKSYLVSQGWSRDKLYAVDFWDKTGTNYNNGPVLSRFVQKVLDETGAKKVDIVAHSMGGANTLYYIKNLDGGNKVANVVTLGGANRLTTGKALPGTDPNQKILYTSIYSSADMIVMNYLSRLDGARNVQIHGVGHIGLLYSSQVNSLIKEGLNGGGQNTN
Palanichamy Esakkiraj et al. (2017) β€” Molecular characterization and application of lipase from Bacillus sp. PU1 and investigation of structural changes based on pH and temperature using MD simulation
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2017.04.111 β†—  Β· Stability - Incubation
48 measurements ▼
Database ID
UniParc: UPI000005FEB5 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3) pLysS

Experimental Data (48 measurements)

48 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethyl Acetate 10% 110 61 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Chloroform 10% 133 129 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Chloroform 10% 114 107 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Chloroform 10% 128 118 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Chloroform 10% 114 104 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Chloroform 10% 107 85 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 10% 110 107 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 10% 133 118 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 10% 114 118 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 10% 128 109 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 10% 114 103 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 10% 107 92 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Chloroform 10% 110 106 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethyl Acetate 10% 133 90 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethyl Acetate 10% 114 65 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethyl Acetate 10% 128 76 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethyl Acetate 10% 114 79 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethyl Acetate 10% 107 43 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase 1-Butanol 10% 110 46 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase 1-Butanol 10% 133 76 U Incubation: ?, Assay: 60 mM Tris-HCl, 6 mM CaCl2, 125 mM NaCl Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in U), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, temperature, solution and mixing data for assay taken from reference 11
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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