β-lactamase (gene ampC) from Escherichia coli K12

Enzyme Description

Species

Extremophile

EC Number

3.5.2.6 ↗

Sequence

Length: 377 amino acids

MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGMAVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLSDPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGYREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYWQTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGATGGFGSYVAFIPEKELGIVMLANKNYPNPARVDAAWQILNALQ

Hiroko Tokunaga et al. (2017) — Reversible Activation of Halophilic β-lactamase from Methanol-Induced Inactive Form: Contrast to Irreversible Inactivation of Non-Halophilic Counterpart

The Protein Journal · doi:10.1007/s10930-017-9715-0 ↗ · Activity - Classical

4 measurements ▼

Database ID

UniProt: P00811 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (4 measurements)

4 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity after incubation (5 hours at 25°C), measured by absorbance spectrophotometry (reaction product absorbance measurement, 486 nm) in the presence of organic solvent	Methanol	10%	100	60	%	50 mM Tris-HCl buffer	8	Room temperature	0.1 mM Nitrocefin	5‑nitro‑3‑oxo‑3H‑phenoxazin‑7‑ylacetamido‑methyl‑thiazolidine‑2‑carboxylate (ring-opened β-lactam form)	—	—	Classical aqueous control (in %)
Activity - Classical	Activity after incubation (5 hours at 25°C), measured by absorbance spectrophotometry (reaction product absorbance measurement, 486 nm) in the presence of organic solvent	Methanol	20%	100	52	%	50 mM Tris-HCl buffer	8	Room temperature	0.1 mM Nitrocefin	5‑nitro‑3‑oxo‑3H‑phenoxazin‑7‑ylacetamido‑methyl‑thiazolidine‑2‑carboxylate (ring-opened β-lactam form)	—	—	Classical aqueous control (in %)
Activity - Classical	Activity after incubation (5 hours at 25°C), measured by absorbance spectrophotometry (reaction product absorbance measurement, 486 nm) in the presence of organic solvent	Methanol	40%	100	12	%	50 mM Tris-HCl buffer	8	Room temperature	0.1 mM Nitrocefin	5‑nitro‑3‑oxo‑3H‑phenoxazin‑7‑ylacetamido‑methyl‑thiazolidine‑2‑carboxylate (ring-opened β-lactam form)	—	—	Classical aqueous control (in %)
Activity - Classical	Activity after incubation (5 hours at 25°C), measured by absorbance spectrophotometry (reaction product absorbance measurement, 486 nm) in the presence of organic solvent	Methanol	60%	100	6	%	50 mM Tris-HCl buffer	8	Room temperature	0.1 mM Nitrocefin	5‑nitro‑3‑oxo‑3H‑phenoxazin‑7‑ylacetamido‑methyl‑thiazolidine‑2‑carboxylate (ring-opened β-lactam form)	—	—	Classical aqueous control (in %)

Activity - Classical	Activity after incubation (5 hours at 25°C), measured by absorbance spectrophotometry (reaction product absorbance measurement, 486 nm) in the presence of organic solvent	Methanol	10%	100	60	%	50 mM Tris-HCl buffer	8	Room temperature	0.1 mM Nitrocefin	5‑nitro‑3‑oxo‑3H‑phenoxazin‑7‑ylacetamido‑methyl‑thiazolidine‑2‑carboxylate (ring-opened β-lactam form)	—	—	Classical aqueous control (in %)
Activity - Classical	Activity after incubation (5 hours at 25°C), measured by absorbance spectrophotometry (reaction product absorbance measurement, 486 nm) in the presence of organic solvent	Methanol	20%	100	52	%	50 mM Tris-HCl buffer	8	Room temperature	0.1 mM Nitrocefin	5‑nitro‑3‑oxo‑3H‑phenoxazin‑7‑ylacetamido‑methyl‑thiazolidine‑2‑carboxylate (ring-opened β-lactam form)	—	—	Classical aqueous control (in %)
Activity - Classical	Activity after incubation (5 hours at 25°C), measured by absorbance spectrophotometry (reaction product absorbance measurement, 486 nm) in the presence of organic solvent	Methanol	40%	100	12	%	50 mM Tris-HCl buffer	8	Room temperature	0.1 mM Nitrocefin	5‑nitro‑3‑oxo‑3H‑phenoxazin‑7‑ylacetamido‑methyl‑thiazolidine‑2‑carboxylate (ring-opened β-lactam form)	—	—	Classical aqueous control (in %)
Activity - Classical	Activity after incubation (5 hours at 25°C), measured by absorbance spectrophotometry (reaction product absorbance measurement, 486 nm) in the presence of organic solvent	Methanol	60%	100	6	%	50 mM Tris-HCl buffer	8	Room temperature	0.1 mM Nitrocefin	5‑nitro‑3‑oxo‑3H‑phenoxazin‑7‑ylacetamido‑methyl‑thiazolidine‑2‑carboxylate (ring-opened β-lactam form)	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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