Chymotrypsin from Helicoverpa armigera

Enzyme Description

Species

Extremophile

EC Number

3.4.21.1 ↗

Sequence

Length: 295 amino acids

MKFVALTLLALAAVASARNVHLEDSIDLEDITAWGYLTKFGIPEAEKIRSAEEASSASRIVGGSLSSLGQIPYQAGLVIDLSGGQAVCGGSLISASRVLTAAHCWFDGQNQAWRFTVVLGSTTLFSGGTRIATSNVVMHGSWTPSLIRNDVAVIRLGTNVGTSNTIAIIALPSGSQINENFAGETALASGFGLTSDSGSISSNQALSHVNLPVITNAVCRSSFPLLIQDSNICTSGANGRSTCRGDSGGPLAVTRNSRPLLIGITSFGSARGCQVGSPAAFARVTSYISWINGQL

Shaik Mohammad Akbar et al. (2016) — Alkaline serine proteases from Helicoverpa armigera : potential candidates for industrial applications

Archives of Insect Biochemistry and Physiology · doi:10.1002/arch.21367 ↗ · Activity - Classical

12 measurements ▼

Database ID

UniProt: D7RZZ6 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, insect Helicoverpa armigera

Experimental Data (12 measurements)

12 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	5%	100.0	114.39	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline , Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	5%	100.0	105.77	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline , Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	1-Propanol	5%	100.0	97.33	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline , Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	1-Butanol	5%	100.0	105.3	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline , Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	5%	100.0	112.55	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline , Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	5%	100.0	111.62	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline , Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100.0	118.98	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline , Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Dimethylformamide (DMF)	5%	100.0	110.17	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline , Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Benzene	5%	100.0	112.59	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline , Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Toluene	5%	100.0	108.69	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline , Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Diethyl Ether	5%	100.0	90.0	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline , Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Chloroform	5%	100.0	106.06	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline , Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	5%	100.0	114.39	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline, Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	5%	100.0	105.77	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline, Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	1-Propanol	5%	100.0	97.33	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline, Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	1-Butanol	5%	100.0	105.3	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline, Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	5%	100.0	112.55	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline, Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	5%	100.0	111.62	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline, Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100.0	118.98	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline, Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Dimethylformamide (DMF)	5%	100.0	110.17	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline, Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Benzene	5%	100.0	112.59	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline, Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Toluene	5%	100.0	108.69	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline, Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Diethyl Ether	5%	100.0	90.0	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline, Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) in the presence of organic solvent	Chloroform	5%	100.0	106.06	%	100 mM glycine–NaOH buffer	10	37°C	0.1 mM N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide	p-Nitroaniline, Suc-Ala-Ala-Pro-Phe	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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