Lactonase/phosphotriesterase (gene SsoPox) from Sulfolobus solfataricus

Enzyme Description

Species
Sulfolobus solfataricus β†—
Extremophile
Yes "hyperthermophilic" organism
EC Number
3.1.8.1 β†—

Sequence

Length: 314 amino acids
MRIPLVGKDSIESKDIGFTLIHEHLRVFSEAVRQQWPHLYNEDEEFRNAVNEVKRAMQFGVKTIVDPTVMGLGRDIRFMEKVVKATGINLVAGTGIYIYIDLPFYFLNRSIDEIADLFIHDIKEGIQGTLNKAGFVKIAADEPGITKDVEKVIRAAAIANKETKVPIITHSNAHNNTGLEQQRILTEEGVDPGKILIGHLGDTDNIDYIKKIADKGSFIGLDRYGLDLFLPVDKRNETTLRLIKDGYSDKIMISHDYCCTIDWGTAKPEYKPKLAPRWSITLIFEDTIPFLKRNGVNEEVIATIFKENPKKFFS
Benjamin RΓ©my et al. (2016) β€” Harnessing hyperthermostable lactonase from Sulfolobus solfataricus for biotechnological applications
Scientific Reports  Β· doi:10.1038/srep37780 β†—  Β· Stability - Incubation
16 measurements ▼
Database ID
UniProt: Q97VT7 β†—
Sequence Annotation
Inferred - from protein name (1 mutation, W263I, compared to UniProt sequence)
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (16 measurements)

16 measurements
Mutation Property Assay Solvent Solvent Volume Aqueous ReferenceWT Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
W263I Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 100% 100 β€” 86 % Incubation: /, Assay: 50 mM HEPES buffer, 150 mM NaCl Incubation: /, Assay: 8 Incubation: Room temperature, Assay: 25Β°C 1 mM Paraoxon diethyl phosphate , p-Nitrophenol β€” Yes, "30s vortex after adding solvent for incubation" Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
W263I Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 100% 100 β€” 94 % Incubation: /, Assay: 50 mM HEPES buffer, 150 mM NaCl Incubation: /, Assay: 8 Incubation: Room temperature, Assay: 25Β°C 1 mM Paraoxon diethyl phosphate , p-Nitrophenol β€” Yes, "30s vortex after adding solvent for incubation" Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
W263I Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 2-Butanone 100% 100 β€” 102 % Incubation: /, Assay: 50 mM HEPES buffer, 150 mM NaCl Incubation: /, Assay: 8 Incubation: Room temperature, Assay: 25Β°C 1 mM Paraoxon diethyl phosphate , p-Nitrophenol β€” Yes, "30s vortex after adding solvent for incubation" Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
W263I Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Butyl Acetate 100% 100 β€” 107 % Incubation: /, Assay: 50 mM HEPES buffer, 150 mM NaCl Incubation: /, Assay: 8 Incubation: Room temperature, Assay: 25Β°C 1 mM Paraoxon diethyl phosphate , p-Nitrophenol β€” Yes, "30s vortex after adding solvent for incubation" Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
W263I Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Chloroform 100% 100 β€” 89 % Incubation: /, Assay: 50 mM HEPES buffer, 150 mM NaCl Incubation: /, Assay: 8 Incubation: Room temperature, Assay: 25Β°C 1 mM Paraoxon diethyl phosphate , p-Nitrophenol β€” Yes, "30s vortex after adding solvent for incubation" Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
W263I Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dichloromethane 100% 100 β€” 95 % Incubation: /, Assay: 50 mM HEPES buffer, 150 mM NaCl Incubation: /, Assay: 8 Incubation: Room temperature, Assay: 25Β°C 1 mM Paraoxon diethyl phosphate , p-Nitrophenol β€” Yes, "30s vortex after adding solvent for incubation" Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
W263I Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Diethyl Ether 100% 100 β€” 89 % Incubation: /, Assay: 50 mM HEPES buffer, 150 mM NaCl Incubation: /, Assay: 8 Incubation: Room temperature, Assay: 25Β°C 1 mM Paraoxon diethyl phosphate , p-Nitrophenol β€” Yes, "30s vortex after adding solvent for incubation" Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
W263I Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 100% 100 β€” 89 % Incubation: /, Assay: 50 mM HEPES buffer, 150 mM NaCl Incubation: /, Assay: 8 Incubation: Room temperature, Assay: 25Β°C 1 mM Paraoxon diethyl phosphate , p-Nitrophenol β€” Yes, "30s vortex after adding solvent for incubation" Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
W263I Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethyl Acetate 100% 100 β€” 102 % Incubation: /, Assay: 50 mM HEPES buffer, 150 mM NaCl Incubation: /, Assay: 8 Incubation: Room temperature, Assay: 25Β°C 1 mM Paraoxon diethyl phosphate , p-Nitrophenol β€” Yes, "30s vortex after adding solvent for incubation" Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
W263I Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 100% 100 β€” 87 % Incubation: /, Assay: 50 mM HEPES buffer, 150 mM NaCl Incubation: /, Assay: 8 Incubation: Room temperature, Assay: 25Β°C 1 mM Paraoxon diethyl phosphate , p-Nitrophenol β€” Yes, "30s vortex after adding solvent for incubation" Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
W263I Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 100% 100 β€” 91 % Incubation: /, Assay: 50 mM HEPES buffer, 150 mM NaCl Incubation: /, Assay: 8 Incubation: Room temperature, Assay: 25Β°C 1 mM Paraoxon diethyl phosphate , p-Nitrophenol β€” Yes, "30s vortex after adding solvent for incubation" Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
W263I Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methoxypropanol 100% 100 β€” 111 % Incubation: /, Assay: 50 mM HEPES buffer, 150 mM NaCl Incubation: /, Assay: 8 Incubation: Room temperature, Assay: 25Β°C 1 mM Paraoxon diethyl phosphate , p-Nitrophenol β€” Yes, "30s vortex after adding solvent for incubation" Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
W263I Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methylcyclohexane 100% 100 β€” 255 % Incubation: /, Assay: 50 mM HEPES buffer, 150 mM NaCl Incubation: /, Assay: 8 Incubation: Room temperature, Assay: 25Β°C 1 mM Paraoxon diethyl phosphate , p-Nitrophenol β€” Yes, "30s vortex after adding solvent for incubation" Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
W263I Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Petroleum Ether 100% 100 β€” 78 % Incubation: /, Assay: 50 mM HEPES buffer, 150 mM NaCl Incubation: /, Assay: 8 Incubation: Room temperature, Assay: 25Β°C 1 mM Paraoxon diethyl phosphate , p-Nitrophenol β€” Yes, "30s vortex after adding solvent for incubation" Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
W263I Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Toluene 100% 100 β€” 74 % Incubation: /, Assay: 50 mM HEPES buffer, 150 mM NaCl Incubation: /, Assay: 8 Incubation: Room temperature, Assay: 25Β°C 1 mM Paraoxon diethyl phosphate , p-Nitrophenol β€” Yes, "30s vortex after adding solvent for incubation" Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase
W263I Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Xylene 100% 100 β€” 24 % Incubation: /, Assay: 50 mM HEPES buffer, 150 mM NaCl Incubation: /, Assay: 8 Incubation: Room temperature, Assay: 25Β°C 1 mM Paraoxon diethyl phosphate , p-Nitrophenol β€” Yes, "30s vortex after adding solvent for incubation" Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase

Mutations in this dataset (1)

W263I

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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