Transaminase from Vulcanisaeta moutnovskia

Enzyme Description

Species

Vulcanisaeta moutnovskia ↗

Extremophile

Yes "hyperthermophilic" organism

EC Number

2.6.1.42 ↗

Sequence

Length: 314 amino acids

MIMLNKEPIVQHLMKYVWVNGKLIPEKEATIPILTHALHYGTSIFEGIRAYWNSDNNNLYVFRARDHYVRFHDSAKIMSIKVGYSVDELIDATVELLRANDVHEDVYIRPITFVSASTVNLDIRNLDVTTAIITVPFGHYLEPKGIKAKVVSWLRVHNSMFPMKAKVSGIYVNSVIAIIDAKVSGFDEAILLNRDGYVAEGSGENIFIIKDGILYTPPVYDSILEGITRDTVITIARDLGLTVTEKRITREELYTADEVFFTGTAAEVTPVVNIDGRVIGNGEPGPIALKVRSYYMDVVHGRVSKYKNWLTPIY

Tatiana N. Stekhanova et al. (2017) — A Novel highly thermostable branched-chain amino acid aminotransferase from the crenarchaeon Vulcanisaeta moutnovskia

Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2016.10.002 ↗ · Activity - Classical

4 measurements ▼

Database ID

UniProt: F0QW25 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli DLT1270

Experimental Data (4 measurements)

4 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (alanine dehydrogenase assay, NADH absorbance measurement, with reaction catalyzed by an alanine dehydrogenase from Bacillus cereus) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	3.7	2.6	%	50 mM Tris-HCl buffer, 100 mM NaCl	8	65°C	20 mM L-ornithine, 40 mM Pyruvate	Glutamate-5-semialdehyde , L-Alanine	60 µM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (alanine dehydrogenase assay, NADH absorbance measurement, with reaction catalyzed by an alanine dehydrogenase from Bacillus cereus) in the presence of organic solvent	Dimethylformamide (DMF)	15%	3.7	1.4	%	50 mM Tris-HCl buffer, 100 mM NaCl	8	65°C	20 mM L-ornithine, 40 mM Pyruvate	Glutamate-5-semialdehyde , L-Alanine	60 µM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (alanine dehydrogenase assay, NADH absorbance measurement, with reaction catalyzed by an alanine dehydrogenase from Bacillus cereus) in the presence of organic solvent	Methanol	15%	3.7	2.4	%	50 mM Tris-HCl buffer, 100 mM NaCl	8	65°C	20 mM L-ornithine, 40 mM Pyruvate	Glutamate-5-semialdehyde , L-Alanine	60 µM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (alanine dehydrogenase assay, NADH absorbance measurement, with reaction catalyzed by an alanine dehydrogenase from Bacillus cereus) in the presence of organic solvent	Acetonitrile	15%	3.7	1.2	%	50 mM Tris-HCl buffer, 100 mM NaCl	8	65°C	20 mM L-ornithine, 40 mM Pyruvate	Glutamate-5-semialdehyde , L-Alanine	60 µM PLP	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (alanine dehydrogenase assay, NADH absorbance measurement, with reaction catalyzed by an alanine dehydrogenase from Bacillus cereus) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	3.7	2.6	%	50 mM Tris-HCl buffer, 100 mM NaCl	8	65°C	20 mM L-ornithine, 40 mM Pyruvate	Glutamate-5-semialdehyde, L-Alanine	60 µM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (alanine dehydrogenase assay, NADH absorbance measurement, with reaction catalyzed by an alanine dehydrogenase from Bacillus cereus) in the presence of organic solvent	Dimethylformamide (DMF)	15%	3.7	1.4	%	50 mM Tris-HCl buffer, 100 mM NaCl	8	65°C	20 mM L-ornithine, 40 mM Pyruvate	Glutamate-5-semialdehyde, L-Alanine	60 µM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (alanine dehydrogenase assay, NADH absorbance measurement, with reaction catalyzed by an alanine dehydrogenase from Bacillus cereus) in the presence of organic solvent	Methanol	15%	3.7	2.4	%	50 mM Tris-HCl buffer, 100 mM NaCl	8	65°C	20 mM L-ornithine, 40 mM Pyruvate	Glutamate-5-semialdehyde, L-Alanine	60 µM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (alanine dehydrogenase assay, NADH absorbance measurement, with reaction catalyzed by an alanine dehydrogenase from Bacillus cereus) in the presence of organic solvent	Acetonitrile	15%	3.7	1.2	%	50 mM Tris-HCl buffer, 100 mM NaCl	8	65°C	20 mM L-ornithine, 40 mM Pyruvate	Glutamate-5-semialdehyde, L-Alanine	60 µM PLP	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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