Esterase (gene Pf_Est) from Pyrococcus furiosus COM1
Enzyme Description
Species
Extremophile
Yes
"hyperthermophilic" organism
EC Number
Sequence
ASITFEMFIPQLNTTRRIWIYLPPDYNYSTKRYPVLYMFDGQNLFDAATSFAGEWGVDEALEKLYKEKNFSIIVVGIDNGGDRRIDEYAPWVNRDYRRGGLGNATVKFIVETLKPYIDAHYRTDPEKTGIMGSSLGGLMAIYAGFSYPEVFRYVGAMSSAFWFNPEIYDFVREAKKGPEKIYIDWGTNEGRNPKAFSESNEKMVKILKEKGYREEFNLKVVIDKGGLHNEYYWGKRFPQAVLWLFEE
F. Mandelli et al. (2016)
β Characterization and Low-Resolution Structure of an Extremely Thermostable Esterase of Potential Biotechnological Interest from Pyrococcus furiosus
Molecular Biotechnology
Β· doi:10.1007/s12033-016-9975-5 β
Β· Stability - Incubation
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Database ID
UniProt: I6UZ00 β
Sequence Annotation
Explicit - Provided GenBank Accession Number (159 first residues from UniProt sequence absent from the mature protein, and residues AS added at N-terminal end)
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (12 measurements)
12 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Isopropanol | 5% | 100.0 | 90.7 | % | Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v | Incubation: 8, Assay: 8 | Incubation: 60Β°C, Assay: 80Β°C | 2 mM p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Isopropanol | 50% | 100.0 | 52.7 | % | Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v | Incubation: 8, Assay: 8 | Incubation: 60Β°C, Assay: 80Β°C | 2 mM p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Isobutanol | 5% | 100.0 | 118.3 | % | Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v | Incubation: 8, Assay: 8 | Incubation: 60Β°C, Assay: 80Β°C | 2 mM p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Isobutanol | 50% | 100.0 | 63.9 | % | Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v | Incubation: 8, Assay: 8 | Incubation: 60Β°C, Assay: 80Β°C | 2 mM p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Methanol | 5% | 100.0 | 115.2 | % | Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v | Incubation: 8, Assay: 8 | Incubation: 60Β°C, Assay: 80Β°C | 2 mM p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Methanol | 50% | 100.0 | 39.5 | % | Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v | Incubation: 8, Assay: 8 | Incubation: 60Β°C, Assay: 80Β°C | 2 mM p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Ethanol | 5% | 100.0 | 102.8 | % | Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v | Incubation: 8, Assay: 8 | Incubation: 60Β°C, Assay: 80Β°C | 2 mM p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Ethanol | 50% | 100.0 | 51.5 | % | Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v | Incubation: 8, Assay: 8 | Incubation: 60Β°C, Assay: 80Β°C | 2 mM p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | 1-Hexanol | 5% | 100.0 | 99.8 | % | Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v | Incubation: 8, Assay: 8 | Incubation: 60Β°C, Assay: 80Β°C | 2 mM p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | 1-Hexanol | 50% | 100.0 | 111.2 | % | Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v | Incubation: 8, Assay: 8 | Incubation: 60Β°C, Assay: 80Β°C | 2 mM p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Dichloromethane | 5% | 100.0 | 213.0 | % | Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v | Incubation: 8, Assay: 8 | Incubation: 60Β°C, Assay: 80Β°C | 2 mM p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Dichloromethane | 50% | 100.0 | 369.0 | % | Incubation: 50 mM Tris-HCl, arabic gum 0.1 % w/v, TritonX 100 0.4 % w/v, Assay: 36 mM Tris-HCl,20% isopropanol, arabic gum 0.07 % w/v, TritonX 100 0.3 % w/v | Incubation: 8, Assay: 8 | Incubation: 60Β°C, Assay: 80Β°C | 2 mM p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.