Lipase from Pseudomonas sp. strain ASM3

Enzyme Description

Species
Pseudomonas sp. AMS3 β†—
Extremophile
No but "thermotolerant" enzyme and organism from Antarctica (but no explicit psychrophilic annotation)
EC Number
3.1.1.3 β†—

Sequence

Length: 388 amino acids
ASLRANDAPIVLLHGFTGWGREEMFGFKYWGGVRGDIEQWLNDNGYRTYTLAVGPLSSNWDRACEAYAQLVGGTVDYGAAHAAKHGHARFGRTYPGLLPELKRGGRIHIIAHSQGGQTARMLVSLLENGSQEEREYAKAHNVSLSPLFEGGHHFVLSVTTIATPHDGTTLVNMVDFTDRFFDLQKAVLEAAAVASNVPYTSQVYDFKLDQWGLRRQPGESFDHYFERLKRSPVWTSTDTARYDLSVSGAEKLNQWVQASPNTYYLSFSTERTYRGALTGNHYPELGMNAFSAVVCAPFLGSYRNPTLGIDDRWLENDGIVNTVSMNGPKRGSSDRIVPYDGTLKKGVWNDMGTYNVDHLEIIGVDPNPSFDIRAFYLRLAEQLASLRP
Wahhida Latip et al. (2016) β€” Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonas sp strain AMS3
PeerJ  Β· doi:10.7717/peerj.2420 β†—  Β· Activity + Stability - Incubation
11 measurements ▼
Database ID
UniProt: A0A0K2CUU1 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (11 measurements)

11 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity + Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 25% 100.0 73.7 % Incubation: 50 Mm phosphate buffer, Assay: 18 mM phosphate buffer, 0.5% v/v CaCl2.H2O Incubation: 8, Assay: 8 Incubation: 50Β°C, Assay: 37Β°C 35% Olive oil free fatty acids , glycerol β€” Assay: 250 rpm Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in the presence of organic solvent Methanol 25% 100.0 88.7 % Incubation: 50 Mm phosphate buffer, Assay: 18 mM phosphate buffer, 0.5% v/v CaCl2.H2O Incubation: 8, Assay: 8 Incubation: 50Β°C, Assay: 37Β°C 35% Olive oil free fatty acids , glycerol β€” Assay: 250 rpm Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in the presence of organic solvent Acetonitrile 25% 100.0 7.9 % Incubation: 50 Mm phosphate buffer, Assay: 18 mM phosphate buffer, 0.5% v/v CaCl2.H2O Incubation: 8, Assay: 8 Incubation: 50Β°C, Assay: 37Β°C 35% Olive oil free fatty acids , glycerol β€” Assay: 250 rpm Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in the presence of organic solvent Ethanol 25% 100.0 35.9 % Incubation: 50 Mm phosphate buffer, Assay: 18 mM phosphate buffer, 0.5% v/v CaCl2.H2O Incubation: 8, Assay: 8 Incubation: 50Β°C, Assay: 37Β°C 35% Olive oil free fatty acids , glycerol β€” Assay: 250 rpm Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in the presence of organic solvent Acetone 25% 100.0 25.8 % Incubation: 50 Mm phosphate buffer, Assay: 18 mM phosphate buffer, 0.5% v/v CaCl2.H2O Incubation: 8, Assay: 8 Incubation: 50Β°C, Assay: 37Β°C 35% Olive oil free fatty acids , glycerol β€” Assay: 250 rpm Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in the presence of organic solvent 1-Propanol 25% 100.0 6.4 % Incubation: 50 Mm phosphate buffer, Assay: 18 mM phosphate buffer, 0.5% v/v CaCl2.H2O Incubation: 8, Assay: 8 Incubation: 50Β°C, Assay: 37Β°C 35% Olive oil free fatty acids , glycerol β€” Assay: 250 rpm Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in the presence of organic solvent Chloroform 25% 100.0 20.6 % Incubation: 50 Mm phosphate buffer, Assay: 18 mM phosphate buffer, 0.5% v/v CaCl2.H2O Incubation: 8, Assay: 8 Incubation: 50Β°C, Assay: 37Β°C 35% Olive oil free fatty acids , glycerol β€” Assay: 250 rpm Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in the presence of organic solvent Benzene 25% 100.0 41.9 % Incubation: 50 Mm phosphate buffer, Assay: 18 mM phosphate buffer, 0.5% v/v CaCl2.H2O Incubation: 8, Assay: 8 Incubation: 50Β°C, Assay: 37Β°C 35% Olive oil free fatty acids , glycerol β€” Assay: 250 rpm Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in the presence of organic solvent Toluene 25% 100.0 29.9 % Incubation: 50 Mm phosphate buffer, Assay: 18 mM phosphate buffer, 0.5% v/v CaCl2.H2O Incubation: 8, Assay: 8 Incubation: 50Β°C, Assay: 37Β°C 35% Olive oil free fatty acids , glycerol β€” Assay: 250 rpm Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in the presence of organic solvent Xylene 25% 100.0 40.0 % Incubation: 50 Mm phosphate buffer, Assay: 18 mM phosphate buffer, 0.5% v/v CaCl2.H2O Incubation: 8, Assay: 8 Incubation: 50Β°C, Assay: 37Β°C 35% Olive oil free fatty acids , glycerol β€” Assay: 250 rpm Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Activity + Stability - Incubation Activity after incubation (30 minutes at 50Β°C) measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in the presence of organic solvent n-Hexane 25% 100.0 116.4 % Incubation: 50 Mm phosphate buffer, Assay: 18 mM phosphate buffer, 0.5% v/v CaCl2.H2O Incubation: 8, Assay: 8 Incubation: 50Β°C, Assay: 37Β°C 35% Olive oil free fatty acids , glycerol β€” Assay: 250 rpm Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control

Visualization : Activity + Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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