Lipase (gene LipBST) from Bacillus stratosphericus L1

Enzyme Description

Species

Extremophile

EC Number

3.1.1.3 ↗

Sequence

Length: 215 amino acids

MKVICFKKKSLQILVALALVIGSMAFIQPKEVKAAAHNPVVMVHGMGGASYNFASIKRYLVSQGWDQNQLFAIDFIDKTGNNLNNGPRLSRFVKDVLAKTGAKKVDIVAHSMGGANTLYYIKNLDGGDKIENVVTLGGANGLVSLRALPGTDPNQKILYTSVYSSADLIVVNSLSRLIGARNVLIHGVGHIGLLTSSQVKGYVKEGLNGGGQNTN

Alisa Gricajeva et al. (2016) — Lipase of Bacillus stratosphericus L1: Cloning, expression and characterization

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2016.07.015 ↗ · Stability - Incubation

6 measurements ▼

Database ID

UniProt: A0A0D5CCJ2 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (6 measurements)

6 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	50%	100	10	%	Incubation: 50 mM Briton-Robinson buffer, Assay: 50 mM Briton-Robinson buffer, 0.1% gum arabic and 0.2% sodium deoxycholate	Incubation: 9, Assay: 9	Incubation: 35°C, Assay: 35°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay measured in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	50%	100	8	%	Incubation: 50 mM Briton-Robinson buffer, Assay: 50 mM Briton-Robinson buffer, 0.1% gum arabic and 0.2% sodium deoxycholate	Incubation: 9, Assay: 9	Incubation: 35°C, Assay: 35°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay measured in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	50%	100	59	%	Incubation: 50 mM Briton-Robinson buffer, Assay: 50 mM Briton-Robinson buffer, 0.1% gum arabic and 0.2% sodium deoxycholate	Incubation: 9, Assay: 9	Incubation: 35°C, Assay: 35°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay measured in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	50%	100	131	%	Incubation: 50 mM Briton-Robinson buffer, Assay: 50 mM Briton-Robinson buffer, 0.1% gum arabic and 0.2% sodium deoxycholate	Incubation: 9, Assay: 9	Incubation: 35°C, Assay: 35°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay measured in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	50%	100	116	%	Incubation: 50 mM Briton-Robinson buffer, Assay: 50 mM Briton-Robinson buffer, 0.1% gum arabic and 0.2% sodium deoxycholate	Incubation: 9, Assay: 9	Incubation: 35°C, Assay: 35°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay measured in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	34	%	Incubation: 50 mM Briton-Robinson buffer, Assay: 50 mM Briton-Robinson buffer, 0.1% gum arabic and 0.2% sodium deoxycholate	Incubation: 9, Assay: 9	Incubation: 35°C, Assay: 35°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation	Activity after incubation (30 minutes at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	50%	100	10	%	Incubation: 50 mM Briton-Robinson buffer, Assay: 50 mM Briton-Robinson buffer, 0.1% gum arabic and 0.2% sodium deoxycholate	Incubation: 9, Assay: 9	Incubation: 35°C, Assay: 35°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay measured in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	50%	100	8	%	Incubation: 50 mM Briton-Robinson buffer, Assay: 50 mM Briton-Robinson buffer, 0.1% gum arabic and 0.2% sodium deoxycholate	Incubation: 9, Assay: 9	Incubation: 35°C, Assay: 35°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay measured in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	50%	100	59	%	Incubation: 50 mM Briton-Robinson buffer, Assay: 50 mM Briton-Robinson buffer, 0.1% gum arabic and 0.2% sodium deoxycholate	Incubation: 9, Assay: 9	Incubation: 35°C, Assay: 35°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay measured in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	50%	100	131	%	Incubation: 50 mM Briton-Robinson buffer, Assay: 50 mM Briton-Robinson buffer, 0.1% gum arabic and 0.2% sodium deoxycholate	Incubation: 9, Assay: 9	Incubation: 35°C, Assay: 35°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay measured in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	50%	100	116	%	Incubation: 50 mM Briton-Robinson buffer, Assay: 50 mM Briton-Robinson buffer, 0.1% gum arabic and 0.2% sodium deoxycholate	Incubation: 9, Assay: 9	Incubation: 35°C, Assay: 35°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay measured in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	34	%	Incubation: 50 mM Briton-Robinson buffer, Assay: 50 mM Briton-Robinson buffer, 0.1% gum arabic and 0.2% sodium deoxycholate	Incubation: 9, Assay: 9	Incubation: 35°C, Assay: 35°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay measured in aqueous phase

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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