Protease (gene Prt1A) from a tannery activated sludge metagenomic library

Enzyme Description

Species

Na (tannery activated sludge metagenomic library)

Extremophile

Yes "alkaliphilic" enzyme

EC Number

3.4.- ↗

Sequence

Length: 380 amino acids

MKKPLGKIVASTALLISVAFSSSIASAAEEANEKYLIGLNEQEAVSEFVEQVEANDEVAILSEEEEVDIELLHEFDTIPVLSVELSPEEVDALELDPAISYTEEDAEVTTMAQSVPWGISRVQAPADHNRGLTGSGEKVAVLDTGISTHPDLNIRGGASFVPGAPSTHDGNAHGTQVAGTIAALNNSIGVLGVAPNAELYAVNVIGASGSGSVSSIAHGLECAGNNDMHVANFSLGSPSPSASLEQAVNSATSRGVLVVAASGNSGAGSISYPARYANAMAVGATDQNNNRASFTQLGAGLDIVAPGVNVQSSYPGSTYASLNATSMATPHVAGAAALVKHKNPSWSNVQIRNHLENTATSLGSTNLYVSGLVKAEAATR

Selvaraju Gayathri Devi et al. (2016) — Expression and characterization of alkaline protease from the metagenomic library of tannery activated sludge

Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2016.05.012 ↗ · Stability - Incubation

10 measurements ▼

Database ID

UniParc: UPI000444E69A ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3) pLysS

Experimental Data (10 measurements)

10 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	Methanol	5%	100.0	111.0	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	Ethanol	5%	100.0	102.0	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	Isopropanol	5%	100.0	146.6	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	n-Hexane	5%	100.0	91.5	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	Toluene	5%	100.0	55.4	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	Methanol	10%	100.0	194.5	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	Ethanol	10%	100.0	168.8	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	Isopropanol	10%	100.0	202.2	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	n-Hexane	10%	100.0	72.2	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	Toluene	10%	100.0	44.4	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase

Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	Methanol	5%	100.0	111.0	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	Ethanol	5%	100.0	102.0	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	Isopropanol	5%	100.0	146.6	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	n-Hexane	5%	100.0	91.5	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	Toluene	5%	100.0	55.4	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	Methanol	10%	100.0	194.5	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	Ethanol	10%	100.0	168.8	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	Isopropanol	10%	100.0	202.2	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	n-Hexane	10%	100.0	72.2	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at ? °C), measured by absorbance spectrophotometry (free amino acid absorbance measurement, 280 nm) in aqueous phase	Toluene	10%	100.0	44.4	%	Incubation: 50 mM glycine-NaOH buffer, Assay: 50 mM glycine-NaOH	Incubation: 11, Assay: 11	Incubation: ?, Assay: 55°C	0.5% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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