Lipase (gene lipPS1) from Pseudomonas synxantha PS1

Enzyme Description

Species
Pseudomonas synxantha PS1 β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 474 amino acids
MGVFDYKNLGTEGSKTLFADAMAITMYSYHNLDNGFAVGYQNNGLGLGLPATLVSALIGSGNAQGVIPGIPWNPDSEKAALDAVQQAGWTPISASTLGYTGKVDARGTFFGEKAAYGTAQVEVLGKYDDAGKLLEIGIGFRGTSGPRESLITDSIGDVISDLLAAFGPKDYAKNYAGEAFGNLLGNVADYASAQGLSGKDVVVSGHSLGGLAVNSMADLSGSKWSGFYTDSNYVAYASPTQSAGDKVLNVGYENDPVFRALDGSSFNLSSLGVHDKPHESSTDNIVSFNDHYASALWNVLPFSILNLPTWVSHLPTGYGDGMTRILESGFYEQMTRDSTVIVANLSDPARANTWVQDLNRNAEAHKGNTFIIGSDGDDLIKGGRGADFIEGGKGNDTIRDSSGHNTFLFSGQFGNDRVIGYQSTDKLVFNDVQGSPDYRDHTKVVGGDTVISFGADSVTLVGVSSLSGEGIVIS
Xianghai Cai et al. (2016) β€” Biodegradation of waste greases and biochemical properties of a novel lipase from Pseudomonas synxantha PS1
Canadian Journal of Microbiology  Β· doi:10.1139/cjm-2015-0641 β†—  Β· Activity - Classical
18 measurements ▼
Database ID
UniProt: A0A0S2KQT6 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (18 measurements)

18 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 25% 100 87 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Benzene 25% 100 46 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetone 25% 100 88 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 25% 100 85 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 25% 100 61 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent n-Hexane 25% 100 74 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent 1-Butanol 25% 100 69 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 25% 100 78 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 25% 100 88 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 15% 100 112 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Benzene 15% 100 63 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetone 15% 100 92 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 15% 100 93 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 15% 100 95 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent n-Hexane 15% 100 83 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent 1-Butanol 15% 100 116 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 15% 100 95 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 15% 100 127 % 45 mM glycine-NaOH buffer, 10% isopropanol/DMSO (Dimethyl Sulfoxide) 9 40Β°C 2.5 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Disagreement between text and figure legend for assay pH

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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