Cutinase (gene cut5a) from Fusarium oxysporum

Enzyme Description

Species
Fusarium oxysporum β†—
Extremophile
No
EC Number
3.1.1.74 β†—

Sequence

Length: 230 amino acids
MKFSIISTLLAATASALPAGQDAAALEARQLGGSITRNDLANGNSGSCPGVIFIYARGSTESGNLGTLGPRVASKLEAKYGKNGVWIQGVGGAYRATLGDNALPRGTSSAAIREMLGHFSDANQKCPDAVLIAGGYSQGAALAAASVTDVDAGIREKIAGVVLFGYTKNLQNRGKIPSYPEDRTKVFCNTGDLVCTGSLIVAAPHLAYQSAASGAAPEFLIQKADAAGAA
Efstratios Nikolaivits et al. (2016) β€” Microbial host selection and periplasmic folding in Escherichia coli affect the biochemical characteristics of a cutinase from Fusarium oxysporum
Protein Expression and Purification  Β· doi:10.1016/j.pep.2016.06.002 β†—  Β· Stability - Incubation
8 measurements ▼
Database ID
UniProt: X0BTD8 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli Origami 2 (DE3)

Experimental Data (8 measurements)

8 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (20 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 80% 100 17 % Incubation: 20 mM Tris-HCl buffer, Assay: 100 mM phosphate-citrate buffer Incubation: 8, Assay: 6 Incubation: 20Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "under shaking" Water-incubated control (in %), assay measured in aqueous phase
Stability - Incubation Activity after incubation (20 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 80% 100 51 % Incubation: 20 mM Tris-HCl buffer, Assay: 100 mM phosphate-citrate buffer Incubation: 8, Assay: 6 Incubation: 20Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "under shaking" Water-incubated control (in %), assay measured in aqueous phase
Stability - Incubation Activity after incubation (20 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase 1-Propanol 80% 100 40 % Incubation: 20 mM Tris-HCl buffer, Assay: 100 mM phosphate-citrate buffer Incubation: 8, Assay: 6 Incubation: 20Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "under shaking" Water-incubated control (in %), assay measured in aqueous phase
Stability - Incubation Activity after incubation (20 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase 1-Butanol 80% 100 5 % Incubation: 20 mM Tris-HCl buffer, Assay: 100 mM phosphate-citrate buffer Incubation: 8, Assay: 6 Incubation: 20Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "under shaking" Water-incubated control (in %), assay measured in aqueous phase
Stability - Incubation Activity after incubation (20 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 80% 100 22 % Incubation: 20 mM Tris-HCl buffer, Assay: 100 mM phosphate-citrate buffer Incubation: 8, Assay: 6 Incubation: 20Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "under shaking" Water-incubated control (in %), assay measured in aqueous phase
Stability - Incubation Activity after incubation (20 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isooctane 80% 100 187 % Incubation: 20 mM Tris-HCl buffer, Assay: 100 mM phosphate-citrate buffer Incubation: 8, Assay: 6 Incubation: 20Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "under shaking" Water-incubated control (in %), assay measured in aqueous phase
Stability - Incubation Activity after incubation (20 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Decane 80% 100 163 % Incubation: 20 mM Tris-HCl buffer, Assay: 100 mM phosphate-citrate buffer Incubation: 8, Assay: 6 Incubation: 20Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "under shaking" Water-incubated control (in %), assay measured in aqueous phase
Stability - Incubation Activity after incubation (20 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Toluene 80% 100 137 % Incubation: 20 mM Tris-HCl buffer, Assay: 100 mM phosphate-citrate buffer Incubation: 8, Assay: 6 Incubation: 20Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Yes, incubation "under shaking" Water-incubated control (in %), assay measured in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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