Esterase (gene Lip10) from Monascus ruber M7

Enzyme Description

Species
Monascus ruber M7 β†—
Extremophile
No but "cold-active" and "organic solvent‐tolerant" enzyme
EC Number
3.1.1.1 β†—

Sequence

Length: 348 amino acids
MADSKNHEALNPIHPSVLPHLDPTFVQLYNENVANTPNRPIDLSVLRSKYSVLYSYGTGPAPDCARVWETEVPAFEGALIRVRVYEPASKGPWPVHVDFHGGGWGLGDLDTESHICKHLCVKADVAVIDVDYRLVPEYPFPIGIQDSFAALQYIHQHGAEEFNIDPTRISIGGVSAGGNIALVCAHLARDEKLPLRLVVAGTPTIDDISRYASAQDSPFRSMQEMEHAPTLNWARLKWFDNLKWQSLSADPAEKEKQMAAVKWYANALNAPDFSHLPKTVVYTAGCDPLRDEGEAYARRLVEAGNEVLQLRFLGVPHPFMHMDKDLSQARDFIDRMAFEIKVALHGKR
Hailun Guo et al. (2016) β€” Cloning, expression and characterization of a novel cold-active and organic solvent-tolerant esterase from Monascus ruber M7
Extremophiles  Β· doi:10.1007/s00792-016-0835-9 β†—  Β· Stability - Incubation
8 measurements ▼
Database ID
UniProt: A0A0S2CGG8 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (8 measurements)

8 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (24 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Toluene 50% 100 216 % Incubation: 50 mM Tris–HCl buffer, Assay: 50 mM Tris–HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 20Β°C, Assay: 40Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase n-Hexane 50% 100 212 % Incubation: 50 mM Tris–HCl buffer, Assay: 50 mM Tris–HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 20Β°C, Assay: 40Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase n-Heptane 50% 100 198 % Incubation: 50 mM Tris–HCl buffer, Assay: 50 mM Tris–HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 20Β°C, Assay: 40Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Chloroform 50% 100 184 % Incubation: 50 mM Tris–HCl buffer, Assay: 50 mM Tris–HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 20Β°C, Assay: 40Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Methanol 50% 100 54 % Incubation: 50 mM Tris–HCl buffer, Assay: 50 mM Tris–HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 20Β°C, Assay: 40Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Ethanol 50% 100 71 % Incubation: 50 mM Tris–HCl buffer, Assay: 50 mM Tris–HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 20Β°C, Assay: 40Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Acetonitrile 50% 100 52 % Incubation: 50 mM Tris–HCl buffer, Assay: 50 mM Tris–HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 20Β°C, Assay: 40Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100 61 % Incubation: 50 mM Tris–HCl buffer, Assay: 50 mM Tris–HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 20Β°C, Assay: 40Β°C 0.4 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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