Lipase (gene LipSM) from Stenotrophomonas maltophilia GS11

Enzyme Description

Species
Stenotrophomonas maltophilia GS11 β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 526 amino acids
MRKVLLLLILLIGLAPTAWAAMATVKTTHRTIAGWDGTPLGAFVIEPQDAGGGRYPLLVMPSSWAVPSVEYVGVAQSLAQRGYVVISYSSRGFWESGGSIDIAGPSTVEDVSALIDWALDNTRADPDRIGVSGISYGAGTSLLAAARDPRIKAVAALSGWADLQASLYSNDTPSAQGIALLVAAGLVTGRPGAELATINRNVLAGNYQGAVDSLLPVAAQRSPAASIDEINANQPAVFLANAFNDSLFPPGQLVDFFNRLKGPKQLQLRHGDHALNEALGALGIPNEVYDQVGDWFDHYLKAVANGIDRQPAVQLKSQKGSWSSYPDWQATSKGAVSYGLTAPSGLLLPTGGLAEHGGGTGWNYRIGSGLLTAANSGVAMASGALQMINLPPGAYVPFVGRSAAGVWQGPIQWSAKRLDGAPEVRLTVTPSRANTTLYAYLYAEDVLGNGQLISHKPYTLRGATPGQAKTLDLRLEASSWNLPAGSRLTLVVDTVDLRYAGISQLGGAVTFTSPANAPSVLKVPLH
Mu Li et al. (2016) β€” Cloning and characterization of a novel lipase from Stenotrophomonas maltophilia GS11: The first member of a new bacterial lipase family XVI
Journal of Biotechnology  Β· doi:10.1016/j.jbiotec.2016.04.034 β†—  Β· Stability - Incubation
32 measurements ▼
Database ID
UniProt: S4TNY8 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (32 measurements)

32 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Benzene 20% 100.0 91.3 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 50% 100.0 0.0 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 20% 100.0 23.2 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 50% 100.0 0.0 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 20% 100.0 73.6 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 50% 100.0 0.0 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 20% 100.0 24.8 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100.0 0.0 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 20% 100.0 40.7 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Heptane 50% 100.0 97.4 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Heptane 20% 100.0 100.6 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexane 50% 100.0 91.8 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexane 20% 100.0 95.4 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Toluene 50% 100.0 100.9 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Toluene 20% 100.0 97.6 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Benzene 50% 100.0 89.7 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Benzene 20% 100.0 100.2 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 50% 100.0 0.0 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 20% 100.0 86.8 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 50% 100.0 20.4 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 35Β°C 0.4 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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