Protease from Stenotrophomonas maltophilia FF11
Enzyme Description
Extremophile
No
but "psychrotolerant", "halo-tolerant" enzyme
EC Number
Sequence
MIKKQNLRINVLAAAVLSMTAVGAVHAAGLPTREPVRQASTAQPGAERIIVKYRAGAAAATDRSAKLSTVQSALTRASLSGGTSRASTLGPQVVRKLATGADLIRVQGRLAPAELQRVLKELQADPSVQYAEADVKLRRTELRAGDVQPALVPNDPFYQQNQWHLHNAVGGINAPAAWDVSQGEGIVVAVIDTGILPQHPDLVGNLLEGYDFISDAETSRRPTNDRVPGAQDYGDWVENDNECYDGSLAEDSSWHGTHVAGTVAEQTNNGVGMAGVAYKAKVLPVRVLGKCGGYLSDIADAVVWASGGTVTGIPANTNPAEIINMSLGGSGACGSTYQDAINGAISRGTTVVVAAGNEADNASKYRPASCEGVVTVGATRITGGITYYSNYGTRVDLSGPGGGGSVDGNPGGYIWQTGSNAATTPESGTPGYMGMGGTSMASPHVAAVAALVQSALIAKGKDPLTPAAMRTLLKETARPFPVAIPAATPIGTGILDAKAALAKALEEPCTENCGPVATPLTNKAAVGGLSGAAGSSKLYSFEAAAGKQFSVITYGGTGNVSVYVAEGREPSASDNDAKSTRPGTSETVRVTKPVAATYYIKVVGEAAYNGVSILATQ
Qingling Wang et al. (2016)
β Characterization of a salt-activated protease with temperature-dependent secretion in Stenotrophomonas maltophilia FF11 isolated from frozen Antarctic krill
Journal of Industrial Microbiology and Biotechnology
Β· doi:10.1007/s10295-016-1749-3 β
Β· Activity - Classical
▼
Database ID
UniProt: A0A120H335 β
Sequence Annotation
Explicit - Provided
Protein Source
Purified, bacterium Stenotrophomonas maltophilia FF11
Experimental Data (18 measurements)
18 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | Ethanol | 50% | 100.0 | 89.1 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | Isooctane | 50% | 100.0 | 100.35 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | Isooctane | 30% | 100.0 | 108.3 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | n-Hexane | 50% | 100.0 | 100.5 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | n-Hexane | 30% | 100.0 | 147.4 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | Isopropanol | 50% | 100.0 | 73.1 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | Isopropanol | 30% | 100.0 | 88.2 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | Acetone | 50% | 100.0 | 134.1 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | Acetone | 30% | 100.0 | 110.0 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 30% | 100.0 | 179.2 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | Ethanol | 30% | 100.0 | 104.2 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | 1,4-Dioxane | 50% | 100.0 | 148.4 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | 1,4-Dioxane | 30% | 100.0 | 138.6 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | Methanol | 50% | 100.0 | 99.7 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | Methanol | 30% | 100.0 | 115.7 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 50% | 100.0 | 153.9 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 30% | 100.0 | 189.2 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 440 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 50% | 100.0 | 135.4 | % | 20 mM Tris-HCl | 8.3 | 55Β°C | 1% Azocasein | azopeptides , Peptides | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.