Family 6 carboxylesterase (gene R-est6) from Methylococcus capsulatus (bath)

Enzyme Description

Species
Methylococcus capsulatus (bath) β†—
Extremophile
No
EC Number
3.1.1.1 β†—

Sequence

Length: 336 amino acids
MSTFDRGFARLLLEICRYTYAKAFALAPEEGDALQWIEQQGGKPDPASLEPIRGGDTSVACVFRYPDKNVVAYMGTKTEFDNAVNAKDSIEDWLNNFRAAPVPFKLGKEHLGLDKEVELPGRVHAGFLSELKAVQAKVIDVLSKNGGKDKPLYLTGHSQGGAEATLATVALLAGGFKVAATYTFAAPRAGDRTFADAVPAEFPFHRIEFGDDIVPHVPPTLLSRWVKTLVTVPRHLPLKRFGVPVKVNEFLELSVHDLGYVGVGRLCYGNHESGQLHVDMSVRQEASLFLGRLTRLLRHPEHWAEHHHLAGTRAEVSAGKKGNYTALVSDFPIVEG
Surabhi Soni et al. (2016) β€” Expression, purification and biochemical characterization of a family 6 carboxylesterase from Methylococcus capsulatus (bath)
Protein Expression and Purification  Β· doi:10.1016/j.pep.2016.02.007 β†—  Β· Stability - Incubation
10 measurements ▼
Database ID
UniProt: A0AA35XUM6 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (10 measurements)

10 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (6 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 50% 100 89 % Incubation: 20 mM phosphate buffer, Assay: 50 mM phosphate buffer, 2.5% isopropanol Incubation: 7.5, Assay: 7.5 Incubation: 37Β°C, Assay: 55Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 50% 100 54 % Incubation: 20 mM phosphate buffer, Assay: 50 mM phosphate buffer, 2.5% isopropanol Incubation: 7.5, Assay: 7.5 Incubation: 37Β°C, Assay: 55Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 50% 100 99 % Incubation: 20 mM phosphate buffer, Assay: 50 mM phosphate buffer, 2.5% isopropanol Incubation: 7.5, Assay: 7.5 Incubation: 37Β°C, Assay: 55Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 50% 100 68 % Incubation: 20 mM phosphate buffer, Assay: 50 mM phosphate buffer, 2.5% isopropanol Incubation: 7.5, Assay: 7.5 Incubation: 37Β°C, Assay: 55Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase 1-Butanol 50% 100 68 % Incubation: 20 mM phosphate buffer, Assay: 50 mM phosphate buffer, 2.5% isopropanol Incubation: 7.5, Assay: 7.5 Incubation: 37Β°C, Assay: 55Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase 1-Butanol 50% 100 39 % Incubation: 20 mM phosphate buffer, Assay: 50 mM phosphate buffer, 2.5% isopropanol Incubation: 7.5, Assay: 7.5 Incubation: 37Β°C, Assay: 55Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100 75 % Incubation: 20 mM phosphate buffer, Assay: 50 mM phosphate buffer, 2.5% isopropanol Incubation: 7.5, Assay: 7.5 Incubation: 37Β°C, Assay: 55Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100 36 % Incubation: 20 mM phosphate buffer, Assay: 50 mM phosphate buffer, 2.5% isopropanol Incubation: 7.5, Assay: 7.5 Incubation: 37Β°C, Assay: 55Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 50% 100 50 % Incubation: 20 mM phosphate buffer, Assay: 50 mM phosphate buffer, 2.5% isopropanol Incubation: 7.5, Assay: 7.5 Incubation: 37Β°C, Assay: 55Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 50% 100 54 % Incubation: 20 mM phosphate buffer, Assay: 50 mM phosphate buffer, 2.5% isopropanol Incubation: 7.5, Assay: 7.5 Incubation: 37Β°C, Assay: 55Β°C 0.25 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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