Lipase from Bacillus amyloliquefaciens PS35

Enzyme Description

Species
Bacillus amyloliquefaciens PS35 β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 214 amino acids
MKQIKSKIIAILTVCMLSVISVFAFQPTVSKASSEHNPVVMVHGIGGASFNFAGIKTYLASQGWSRNEMAAIDFLDKTGNNRHNAPRLSTYVKKVLSETGAKKVDIVAHSMGGANTLYYIKNLDGGDKIANVVTLGGANGLVTNRALPGTDPNQKILYTSIYSAADLIVLNPLSRLIGGKNVQIHGVGHIGLLMMSQVNGLIKEGLNGGGQNTN
Palanisamy Kanmani et al. (2015) β€” Gene cloning, expression, and characterization of the Bacillus amyloliquefaciens PS35 lipase
Brazilian Journal of Microbiology  Β· doi:10.1590/S1517-838246420141068 β†—  Β· Activity - Classical
12 measurements ▼
Database ID
UniProt: A0A0A0RGT3 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli DH5alpha

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 10% 100 91 % ? mM phosphate buffer, 0.7 Mg/ml acacia gum, 9% isopropanol, 4% Triton X-100 7 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 10% 100 85 % ? mM phosphate buffer, 0.7 Mg/ml acacia gum, 9% isopropanol, 4% Triton X-100 7 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Hexane 10% 100 112 % ? mM phosphate buffer, 0.7 Mg/ml acacia gum, 9% isopropanol, 4% Triton X-100 7 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Toluene 10% 100 109 % ? mM phosphate buffer, 0.7 Mg/ml acacia gum, 9% isopropanol, 4% Triton X-100 7 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 20% 100 83 % ? mM phosphate buffer, 0.7 Mg/ml acacia gum, 9% isopropanol, 4% Triton X-100 7 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 20% 100 72 % ? mM phosphate buffer, 0.7 Mg/ml acacia gum, 9% isopropanol, 4% Triton X-100 7 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Hexane 20% 100 123 % ? mM phosphate buffer, 0.7 Mg/ml acacia gum, 9% isopropanol, 4% Triton X-100 7 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Toluene 20% 100 118 % ? mM phosphate buffer, 0.7 Mg/ml acacia gum, 9% isopropanol, 4% Triton X-100 7 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 30% 100 69 % ? mM phosphate buffer, 0.7 Mg/ml acacia gum, 9% isopropanol, 4% Triton X-100 7 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 30% 100 58 % ? mM phosphate buffer, 0.7 Mg/ml acacia gum, 9% isopropanol, 4% Triton X-100 7 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Hexane 30% 100 132 % ? mM phosphate buffer, 0.7 Mg/ml acacia gum, 9% isopropanol, 4% Triton X-100 7 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Toluene 30% 100 128 % ? mM phosphate buffer, 0.7 Mg/ml acacia gum, 9% isopropanol, 4% Triton X-100 7 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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