Ξ±-amylase from Thermotoga petrophila RKU-1

Enzyme Description

Species
Thermotoga petrophila RKU-1 β†—
Extremophile
Yes "hyperthermophilic" organism
EC Number
3.2.1.1 β†—

Sequence

Length: 530 amino acids
QSLVSSNPHSNSTNTGDSASNLSINEVKYPVVYEIFIRSLYDSDGDGVGDINGVSQKVDYLRKLGIDAVWFMPFNEAVSYHGYDITDYYNVEKDYGTMEDLENMIQVLHENGIKVIMDLVINHTSDEHPWFKDAVENTTSSPYWDYYIMSLEDHSGQDHWHWKINSKGQKVWYFGLFGYNMPDLNHDSQKVREEVKKIVDFWISKGVDGFRIDAAKHIYGWSWDDGIQESAEYFEWFRDYVLSKKPDAILVGEVFSGNTYDLSLYPIPVFNFALMYSIRNYPEGQDGMIENNWVEESFLFLENHDLHRFFSHLQEHYKKFSESDYEFIKKRAALWYFLIFTLKGSPVIYYGGEIGTRGFKWHGPVYDEPVREPMQWYASGTGEGQTFWTKEVYKNAGITFGNADVDGCIYDDPYDGFSVEEQENDPKSLLNFIRFILNFRKDHDAILNGDQTIFRDWKNLIAFYRESSNEKLLVVLNPDPVWQNSFTFEENMTMILEVDFENFIWNESNVSFSAGESFTVDPMKAYIFKK
Asma Zafar et al. (2015) β€” Cloning, Purification and Characterization of a Highly Thermostable Amylase Gene of Thermotoga petrophila into Escherichia coli
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-015-1912-8 β†—  Β· Stability - Incubation
20 measurements ▼
Database ID
UniProt: A0A059TXD8 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase Isopropanol 30% 100 91 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase Acetone 40% 100 85 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase Acetone 30% 100 84 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase Acetone 20% 100 84 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase Acetone 10% 100 90 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase 1-Butanol 40% 100 85 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase 1-Butanol 30% 100 86 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase 1-Butanol 20% 100 91 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase 1-Butanol 10% 100 94 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase Isopropanol 40% 100 79 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase Methanol 10% 100 100 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase Isopropanol 20% 100 94 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase Isopropanol 10% 100 97 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase Ethanol 40% 100 91 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase Ethanol 30% 100 96 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase Ethanol 20% 100 99 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase Ethanol 10% 100 99 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase Methanol 40% 100 90 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase Methanol 30% 100 95 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 550 nm) in aqueous phase Methanol 20% 100 100 % ? ? Incubation: Room temperature, Assay: ? ? mM Starch Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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