Lipase from Burkholderia cepacia RQ3

Enzyme Description

Species
Burkholderia cepacia RQ3 β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 364 amino acids
MARTMRSRVVAGAVACAMSIAPFAGTTAVMTLATTHAAMAATAPAAGYAATRYPIILVHGLSGTDKYAGVLEYWYGIQEDLQQNGATVYVANLSGFQSDDGPNGRGEQLLAYVKTVLAATGATKVNLVGHSQGGLSSRYVAAVAPDLVASVTTIGTPHRGSEFADFVQDVLAYDPTGLSSSVIAAFVNVFGILTSSSHNANQDALAALQTLTTARAATYNQNYPSAGLGAPGSCQTGAPTETVGGNTHLLYSWAGTAIQPTLSVFGVTGATDTSTLPLVDPANVLDLSTLALFGTGTVMINRGSGQNDGLVSKCSALYGKVLSTNYKWNHLDEINQLLGVRGAYAEDPVAVIRTHANRLKLAGV
Chengjia Xie et al. (2015) β€” A lipase with broad solvent stability from Burkholderia cepacia RQ3: isolation, characteristics and application for chiral resolution of 1-phenylethanol
Bioprocess and Biosystems Engineering  Β· doi:10.1007/s00449-015-1489-1 β†—  Β· Stability - Half-life
15 measurements ▼
Database ID
β€”
Sequence Annotation
Explicit - Provided
Protein Source
Purified, bacterium Burkholderia cepacia RQ3

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Half-life Half-life (in days) at 40Β°C, with residual activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) n-Hexadecane 25% 3 >10 days Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate, 0.1% gum arabic, 0.6% Triton X-100, 10% isopropanol Incubation: 7, Assay: 8 Incubation: 40Β°C, Assay: 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) at 40Β°C, with residual activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) n-Tetradecane 25% 3 >10 days Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate, 0.1% gum arabic, 0.6% Triton X-100, 10% isopropanol Incubation: 7, Assay: 8 Incubation: 40Β°C, Assay: 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) at 40Β°C, with residual activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) n-Dodecane 25% 3 >10 days Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate, 0.1% gum arabic, 0.6% Triton X-100, 10% isopropanol Incubation: 7, Assay: 8 Incubation: 40Β°C, Assay: 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) at 40Β°C, with residual activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) n-Decane 25% 3 9 days Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate, 0.1% gum arabic, 0.6% Triton X-100, 10% isopropanol Incubation: 7, Assay: 8 Incubation: 40Β°C, Assay: 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) at 40Β°C, with residual activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) n-Nonane 25% 3 9 days Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate, 0.1% gum arabic, 0.6% Triton X-100, 10% isopropanol Incubation: 7, Assay: 8 Incubation: 40Β°C, Assay: 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) at 40Β°C, with residual activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) n-Octane 25% 3 7 days Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate, 0.1% gum arabic, 0.6% Triton X-100, 10% isopropanol Incubation: 7, Assay: 8 Incubation: 40Β°C, Assay: 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) at 40Β°C, with residual activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) n-Heptane 25% 3 7 days Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate, 0.1% gum arabic, 0.6% Triton X-100, 10% isopropanol Incubation: 7, Assay: 8 Incubation: 40Β°C, Assay: 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) at 40Β°C, with residual activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) n-Hexane 25% 3 6 days Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate, 0.1% gum arabic, 0.6% Triton X-100, 10% isopropanol Incubation: 7, Assay: 8 Incubation: 40Β°C, Assay: 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) at 40Β°C, with residual activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) 1-Octanol 25% 3 >10 days Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate, 0.1% gum arabic, 0.6% Triton X-100, 10% isopropanol Incubation: 7, Assay: 8 Incubation: 40Β°C, Assay: 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) at 40Β°C, with residual activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) 1-Heptanol 25% 3 10 days Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate, 0.1% gum arabic, 0.6% Triton X-100, 10% isopropanol Incubation: 7, Assay: 8 Incubation: 40Β°C, Assay: 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) at 40Β°C, with residual activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) 1-Hexanol 25% 3 9 days Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate, 0.1% gum arabic, 0.6% Triton X-100, 10% isopropanol Incubation: 7, Assay: 8 Incubation: 40Β°C, Assay: 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) at 40Β°C, with residual activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) Isopropanol 25% 3 >10 days Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate, 0.1% gum arabic, 0.6% Triton X-100, 10% isopropanol Incubation: 7, Assay: 8 Incubation: 40Β°C, Assay: 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) at 40Β°C, with residual activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) Ethanol 25% 3 >10 days Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate, 0.1% gum arabic, 0.6% Triton X-100, 10% isopropanol Incubation: 7, Assay: 8 Incubation: 40Β°C, Assay: 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) at 40Β°C, with residual activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) Dimethylformamide (DMF) 25% 3 7 days Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate, 0.1% gum arabic, 0.6% Triton X-100, 10% isopropanol Incubation: 7, Assay: 8 Incubation: 40Β°C, Assay: 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) at 40Β°C, with residual activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) Dimethyl Sulfoxide (DMSO) 25% 3 6 days Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate, 0.1% gum arabic, 0.6% Triton X-100, 10% isopropanol Incubation: 7, Assay: 8 Incubation: 40Β°C, Assay: 30Β°C 0.3 Mg/ml p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Classical aqueous control (in days)

Visualization : Stability β€” Half-life

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*