Xylanase (gene xynA) from Bacillus licheniformis 9945A

Enzyme Description

Species
Bacillus licheniformis 9945A β†—
Extremophile
No
EC Number
3.2.1.8 β†—

Sequence

Length: 213 amino acids
MFKFKKNFLVGVTAALMSISLFSATASAASPDYWQNWTDGGGTVNAVNGPGGNYSVNWSNTGNFVVGKGWTTGSPSRTINYNAGVWAPNGNGYLALYGWTRSPLIEYYVVDSWGTYRPTGTYKGTVKSDGGTYDIYTTTRYNAPSIDGEKTTFTQYWSVRQTKRPTGSNAKITFSNHVRAWKSHGMNLGSIWSYQVLATEGYQSSGSSNVTVW
Asma Zafar et al. (2015) β€” Cloning, Expression, and Purification of Xylanase Gene from Bacillus licheniformis for Use in Saccharification of Plant Biomass
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-015-1872-z β†—  Β· Stability - Incubation
20 measurements ▼
Database ID
UniParc: UPI0003423E93 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 30% 100 54 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetone 40% 100 42 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetone 30% 100 50 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetone 20% 100 51 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetone 10% 100 59 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Butanol 40% 100 59 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Butanol 30% 100 63 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Butanol 20% 100 68 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Butanol 10% 100 79 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 40% 100 48 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 10% 100 68 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 20% 100 60 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 10% 100 62 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethanol 40% 100 60 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethanol 30% 100 59 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethanol 20% 100 61 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethanol 10% 100 72 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 40% 100 53 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 30% 100 59 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 20% 100 62 % ? ? Incubation: Room temperature, Assay: ? 1% Xylan Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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