Mannan endo-1,4-β-mannosidase (gene Bpman5) from Bacillus pumilus GBSW19

Enzyme Description

Species

Bacillus pumilus GBSW19 ↗

Extremophile

No but "thermostable" enzyme

EC Number

3.2.1.78 ↗

Sequence

Length: 377 amino acids

MKKWTQRVFCFILVVAIWAGWFSLTIKAASYVQTSGTQFTLNNQPFYFAGTNNYYFHYKSKKMVDAVFGDMKAMNLKVIRIWGFHDGAPQENSVLQSSPGVHEESGFQKLDYAIYKAGQEGIKLVIPLVNNCDDFGGMNQYVKWFEAGTHDAFYTDPRIQKAYKNYVRYVLERTNTYTGVQYKDDPAIMTWELANEPRVQSDPTGNILVKWADEMSTWIKSLDHHHLVAVGDEGFFRIPGHEDWFYGGGEGVDWDRLTALSNIDYGTYHLYPDHWNKSAAWGVKWIEDHITRGKSIGKPVVLEEFGVQNQSARPDVYQSWLSAVERLGGAGSQFWILTSIQDDDSLYPDYDGFRIIKGSRESALISEHAKRMNEKNR

Haoyu Zang et al. (2015) — A novel thermostable GH5_7 β-mannanase from Bacillus pumilus GBSW19 and its application in manno-oligosaccharides (MOS) production

Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2015.06.007 ↗ · Stability - Incubation

12 measurements ▼

Database ID

UniProt: W0I643 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (12 measurements)

12 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	10%	100	87	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	10%	100	97	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isopropanol	10%	100	170	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	10%	100	122	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	20%	100	90	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	20%	100	160	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isopropanol	20%	100	155	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	20%	100	140	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	30%	100	92	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	30%	100	92	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isopropanol	30%	100	123	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	30%	100	106	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	10%	100	87	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	10%	100	97	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isopropanol	10%	100	170	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	10%	100	122	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	20%	100	90	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	20%	100	160	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isopropanol	20%	100	155	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	20%	100	140	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	30%	100	92	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	30%	100	92	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isopropanol	30%	100	123	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 37°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	30%	100	106	%	Incubation: ?, Assay: 100 mM Tris-HCl	Incubation: ?, Assay: 7	Incubation: 37°C, Assay: 60°C	5 mg/ml Locust bean gum	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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