Lipase (gene lipG1) form Acinetobacter sp. G1(2013)
Enzyme Description
Sequence
MQRRMLIAVLTSTAMLLSACHDDDHDSYHSGDNIPKNNITNPVVSTPTAYNQTDMSATAGESVVMTYKMLGINNKEVQATALVFTPKTAPPAKGWPIALWAHGTTGVADICAPSRSQLNSYIKAMIEGLLTAGYVVVAPDYEGLGEPANNELHPFLNVKSEAFSITDAVVAARNYLGNKVSNRWMTVGHSQGGHAALSAAQYQSRAQLDYKGTVAVAPASNLQLILLAGESLAAQQTDIKQKIETLASLDTFTALITAGLRNPNPSLQYSQVFKSPTDKIADNAETDCYPSLEQEFGTAMNTYAEDNADDITNYPRTQSNFMTLSPVKKFLDTDSQPLQVKISTPIIIYQGAMDTTVPKVATDTLLSGDAAKNSPITYKTNTTWDHGTAYVLNIPNILIDVQTLMPIQ
Chao Ran et al. (2015)
β A Novel Lipase as Aquafeed Additive for Warm-Water Aquaculture
PLOS ONE
Β· doi:10.1371/journal.pone.0132049 β
Β· Activity - Classical
▼
Database ID
UniProt: A0A0D3S0I2 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (14 measurements)
14 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 10% | 100.0 | 109.5 | % | 50 mM Tris-HCl buffer | 8 | 40Β°C | 0.5 mM p-Nitrophenyl decanoate | Decanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 10% | 100.0 | 98.2 | % | 50 mM Tris-HCl buffer | 8 | 40Β°C | 0.5 mM p-Nitrophenyl decanoate | Decanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Isopropanol | 10% | 100.0 | 100.3 | % | 50 mM Tris-HCl buffer | 8 | 40Β°C | 0.5 mM p-Nitrophenyl decanoate | Decanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | 2-Octanol | 10% | 100.0 | 88.1 | % | 50 mM Tris-HCl buffer | 8 | 40Β°C | 0.5 mM p-Nitrophenyl decanoate | Decanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | n-Heptane | 10% | 100.0 | 109.7 | % | 50 mM Tris-HCl buffer | 8 | 40Β°C | 0.5 mM p-Nitrophenyl decanoate | Decanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Glycerol | 10% | 100.0 | 93.9 | % | 50 mM Tris-HCl buffer | 8 | 40Β°C | 0.5 mM p-Nitrophenyl decanoate | Decanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 114.3 | % | 50 mM Tris-HCl buffer | 8 | 40Β°C | 0.5 mM p-Nitrophenyl decanoate | Decanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 30% | 100.0 | 78.5 | % | 50 mM Tris-HCl buffer | 8 | 40Β°C | 0.5 mM p-Nitrophenyl decanoate | Decanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 30% | 100.0 | 101.7 | % | 50 mM Tris-HCl buffer | 8 | 40Β°C | 0.5 mM p-Nitrophenyl decanoate | Decanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Isopropanol | 30% | 100.0 | 33.8 | % | 50 mM Tris-HCl buffer | 8 | 40Β°C | 0.5 mM p-Nitrophenyl decanoate | Decanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | 2-Octanol | 30% | 100.0 | 41.3 | % | 50 mM Tris-HCl buffer | 8 | 40Β°C | 0.5 mM p-Nitrophenyl decanoate | Decanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | n-Heptane | 30% | 100.0 | 110.9 | % | 50 mM Tris-HCl buffer | 8 | 40Β°C | 0.5 mM p-Nitrophenyl decanoate | Decanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Glycerol | 30% | 100.0 | 75.5 | % | 50 mM Tris-HCl buffer | 8 | 40Β°C | 0.5 mM p-Nitrophenyl decanoate | Decanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 30% | 100.0 | 108.3 | % | 50 mM Tris-HCl buffer | 8 | 40Β°C | 0.5 mM p-Nitrophenyl decanoate | Decanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.