Esterase (gene SAestA) from Salinispora arenicola CNP193

Enzyme Description

Species
Salinispora arenicola CNP193 β†—
Extremophile
No but "organic-solvent tolerant" enzyme
EC Number
3.1.1.1 β†—

Sequence

Length: 324 amino acids
MSEPTNRPGGGMGLHPEAEAYRAARAAAGTPPLYTQTLAEARAADLAAIQAGAGAVEPVHEVRDGYVPGPDGDIPVRVYRPAGAGPLPTLLYFFGGGWTLGSIDTADGICRRLANAAPCQVVTVGYRLAPEHPFPAAVHDCHAALRHVAAHPAEFDADPERLAVGGDSAGGNLAAAVTLLARDGGPRLAAQVLVYPNTDQTIEPPGGEDPLLFNRRSVTWYRSHYLADPADARHPLASPLLADDLAGLPPALVITAEHDPLCAEGERYADRLRAAGVPTVLTRYPGMVHGFFAMPGTFDAGRRAQAAAATFLGTRFDRTAADDG
Yaowei Fang et al. (2015) β€” Discovery a novel organic solvent tolerant esterase from Salinispora arenicola CNP193 through genome mining
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2015.06.045 β†—  Β· Stability - Incubation
16 measurements ▼
Database ID
UniProt: A0A0A1EF45 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (16 measurements)

16 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 day at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 25% 82 113 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 0.75% acetonitrile, 3% Ethanol Incubation: ?, Assay: 9 Incubation: 25Β°C, Assay: 30Β°C 0.075 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %)
Stability - Incubation Activity after incubation (1 day at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 25% 82 103 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 0.75% acetonitrile, 3% Ethanol Incubation: ?, Assay: 9 Incubation: 25Β°C, Assay: 30Β°C 0.075 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %)
Stability - Incubation Activity after incubation (1 day at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Hexanol 25% 82 122 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 0.75% acetonitrile, 3% Ethanol Incubation: ?, Assay: 9 Incubation: 25Β°C, Assay: 30Β°C 0.075 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %)
Stability - Incubation Activity after incubation (1 day at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Benzene 25% 82 111 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 0.75% acetonitrile, 3% Ethanol Incubation: ?, Assay: 9 Incubation: 25Β°C, Assay: 30Β°C 0.075 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %)
Stability - Incubation Activity after incubation (1 day at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Toluene 25% 82 123 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 0.75% acetonitrile, 3% Ethanol Incubation: ?, Assay: 9 Incubation: 25Β°C, Assay: 30Β°C 0.075 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %)
Stability - Incubation Activity after incubation (1 day at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Xylene 25% 82 133 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 0.75% acetonitrile, 3% Ethanol Incubation: ?, Assay: 9 Incubation: 25Β°C, Assay: 30Β°C 0.075 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %)
Stability - Incubation Activity after incubation (1 day at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Hexane 25% 82 150 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 0.75% acetonitrile, 3% Ethanol Incubation: ?, Assay: 9 Incubation: 25Β°C, Assay: 30Β°C 0.075 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %)
Stability - Incubation Activity after incubation (1 day at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Heptane 25% 82 142 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 0.75% acetonitrile, 3% Ethanol Incubation: ?, Assay: 9 Incubation: 25Β°C, Assay: 30Β°C 0.075 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %)
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 25% 16 0 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 0.75% acetonitrile, 3% Ethanol Incubation: ?, Assay: 9 Incubation: 25Β°C, Assay: 30Β°C 0.075 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %)
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 25% 16 0 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 0.75% acetonitrile, 3% Ethanol Incubation: ?, Assay: 9 Incubation: 25Β°C, Assay: 30Β°C 0.075 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %)
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase 1-Hexanol 25% 16 16 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 0.75% acetonitrile, 3% Ethanol Incubation: ?, Assay: 9 Incubation: 25Β°C, Assay: 30Β°C 0.075 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %)
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Benzene 25% 16 17 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 0.75% acetonitrile, 3% Ethanol Incubation: ?, Assay: 9 Incubation: 25Β°C, Assay: 30Β°C 0.075 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %)
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Toluene 25% 16 22 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 0.75% acetonitrile, 3% Ethanol Incubation: ?, Assay: 9 Incubation: 25Β°C, Assay: 30Β°C 0.075 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %)
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Xylene 25% 16 26 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 0.75% acetonitrile, 3% Ethanol Incubation: ?, Assay: 9 Incubation: 25Β°C, Assay: 30Β°C 0.075 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %)
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Hexane 25% 16 33 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 0.75% acetonitrile, 3% Ethanol Incubation: ?, Assay: 9 Incubation: 25Β°C, Assay: 30Β°C 0.075 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %)
Stability - Incubation Activity after incubation (7 days at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase n-Heptane 25% 16 35 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 0.75% acetonitrile, 3% Ethanol Incubation: ?, Assay: 9 Incubation: 25Β°C, Assay: 30Β°C 0.075 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 200 rpm Water-incubated control (in %)

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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