Glucoamylase (gene rGlucaM) from Corallococcus sp. EGB

Enzyme Description

Species

Extremophile

EC Number

3.2.1.3 ↗

Sequence

Length: 645 amino acids

MDLYSAARRMARPFRFTNPASYREISLAARGVIGDGSSCALVRPDGVIDWLCFPRFDSPSVFAGILDDANGGITGITPVVWPFESLQRYDPDTNVLETLFRFERKGAIRIIDYMPWTNDPRSSVHEVHRRIECLEGSVELNIVFDPRFDYGASRTRVEREEHGLVARGSGGERLVAVLSGEAEWRPCEALRGQPCRGDTGLQTRIRMGPGERRWMILSWDSDRPEPLAAYRPFDHLRDTRQAWREWAHQFHYEGPWRHHVLRSALALKLLMYGPTGAMVAAPTTSLPEWIGGPRNWDYRFSWVRDSAIAVRATNLLGFQGESREFFYFMHDTLQRGDTLQVMYTLDGAAVPPERELDHLFGFQGSRPVRLGNGARDQFQFDTAGALLDAAYLYERSGGRLPLRTWRLLRSVIQTTARRWSEPDHGIWEPRREMRHNVHSKLMGWLALRRGQHMARLFGETALAQSSADMADVVRADILRNGVDPARKHFVGVYGGNEPDAALLQLPIVGCFRGTDPFILRTLDWLRAELGAGPFLRRYRMDDGVAGPEGGFILCGFWLAEALALANRIQEAEDVFVAHAEASNHLGLLAEEIHPLTREQLGNFPQAFSHLGLISAAARIDRALRLRDEGQSEPPHLLEPEPPSIE

Zhoukun Li et al. (2017) — Cloning, heterologous expression, and enzymatic characterization of a novel glucoamylase GlucaM from Corallococcus sp. strain EGB

Protein Expression and Purification · doi:10.1016/j.pep.2015.06.009 ↗ · Activity - Classical

8 measurements ▼

Database ID

UniProt: A0A0H3WKG6 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21

Experimental Data (8 measurements)

8 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Methanol	10%	100.0	97.6	%	50 mM Tris-HCl buffer	7	50°C	0.5% (w/v) Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Methanol	20%	100.0	72.4	%	50 mM Tris-HCl buffer	7	50°C	0.5% (w/v) Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Ethanol	10%	100.0	98.3	%	50 mM Tris-HCl buffer	7	50°C	0.5% (w/v) Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Ethanol	20%	100.0	87.8	%	50 mM Tris-HCl buffer	7	50°C	0.5% (w/v) Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetonitrile	10%	100.0	112.8	%	50 mM Tris-HCl buffer	7	50°C	0.5% (w/v) Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetonitrile	20%	100.0	78.5	%	50 mM Tris-HCl buffer	7	50°C	0.5% (w/v) Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	103.5	%	50 mM Tris-HCl buffer	7	50°C	0.5% (w/v) Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	54.3	%	50 mM Tris-HCl buffer	7	50°C	0.5% (w/v) Starch	Reducing sugars	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Methanol	10%	100.0	97.6	%	50 mM Tris-HCl buffer	7	50°C	0.5% (w/v) Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Methanol	20%	100.0	72.4	%	50 mM Tris-HCl buffer	7	50°C	0.5% (w/v) Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Ethanol	10%	100.0	98.3	%	50 mM Tris-HCl buffer	7	50°C	0.5% (w/v) Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Ethanol	20%	100.0	87.8	%	50 mM Tris-HCl buffer	7	50°C	0.5% (w/v) Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetonitrile	10%	100.0	112.8	%	50 mM Tris-HCl buffer	7	50°C	0.5% (w/v) Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetonitrile	20%	100.0	78.5	%	50 mM Tris-HCl buffer	7	50°C	0.5% (w/v) Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	103.5	%	50 mM Tris-HCl buffer	7	50°C	0.5% (w/v) Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	54.3	%	50 mM Tris-HCl buffer	7	50°C	0.5% (w/v) Starch	Reducing sugars	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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