Laccase (gene atm) from Agrobacterium sp. S5-1
Enzyme Description
Sequence
MVSTAAWAKTSNSSLPEAAVMETAATQAPVKPTTGPDYNPVVTLNGWTLPFRMNNGVKEFHLVAEPVEREMAEGMTARLWGYNGQSPGPTIEAVEGDRVRIFVTNKLPEHTTIHWHGMILPSGMDGVGGLSQPHIPVGKTFVYEFDLVKSGTFMYHPHSDEMVQMAMGMMGFFVIHPKDPAFMPVDRDFVFLINAFDIDPGSYVPKIMTMTDFNLWSWNSRVFPGIDPLVVSKDDRVRVRVGNLTMTNHPIHMHGYDFEVTCTDGGWVRPEARWPEVSIDIPVGAMRAYEFDAKYVGDWAIHCHKSHHTMNAMGHDVPTFIGVDKKTVTEKIRKVRPEYMPMGTAGMADMGAMEMEIPENTVPMMTGWGPHGPIEMGGMFSVVKVREGISAGDYADPGWYENPPGTQAWEWTGELPDTQKNTDPKTQITPQPMKHG
Wei Si et al. (2015)
β Enzymological Characterization of Atm, the First Laccase from Agrobacterium sp. S5-1, with the Ability to Enhance In Vitro digestibility of Maize Straw
PLOS ONE
Β· doi:10.1371/journal.pone.0128204 β
Β· Stability - Incubation
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Database ID
UniProt: A0A0F6SIA9 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21
Experimental Data (8 measurements)
8 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation absorbance measurement, 420 nm) | Acetone | 30% | 100 | 84 | % | Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer | Incubation: 4.5, Assay: 4.5 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM ABTS | ABTS radical cation reaction products | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation absorbance measurement, 420 nm) | Acetone | 10% | 100 | 104 | % | Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer | Incubation: 4.5, Assay: 4.5 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM ABTS | ABTS radical cation reaction products | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation absorbance measurement, 420 nm) | Dimethyl Sulfoxide (DMSO) | 30% | 100 | 72 | % | Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer | Incubation: 4.5, Assay: 4.5 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM ABTS | ABTS radical cation reaction products | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation absorbance measurement, 420 nm) | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 72 | % | Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer | Incubation: 4.5, Assay: 4.5 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM ABTS | ABTS radical cation reaction products | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation absorbance measurement, 420 nm) | Methanol | 30% | 100 | 95 | % | Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer | Incubation: 4.5, Assay: 4.5 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM ABTS | ABTS radical cation reaction products | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation absorbance measurement, 420 nm) | Methanol | 10% | 100 | 84 | % | Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer | Incubation: 4.5, Assay: 4.5 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM ABTS | ABTS radical cation reaction products | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation absorbance measurement, 420 nm) | Ethanol | 30% | 100 | 96 | % | Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer | Incubation: 4.5, Assay: 4.5 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM ABTS | ABTS radical cation reaction products | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (ABTS radical cation absorbance measurement, 420 nm) | Ethanol | 10% | 100 | 96 | % | Incubation: 100 mM sodium acetate buffer, Assay: 100 mM sodium acetate buffer | Incubation: 4.5, Assay: 4.5 | Incubation: 30Β°C, Assay: 30Β°C | 1 mM ABTS | ABTS radical cation reaction products | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.