C-terminal truncated α-amylase from Bacillus subtilis FP-133

Enzyme Description

Species

Extremophile

EC Number

3.2.1.1 ↗

Sequence

Length: 516 amino acids

SVKNGTILHAWNWSFNTLTQNMKEIRDAGYAAIQTSPINQVKEGNQGDKSMRNWYWLYQPTSYQIGNRYLGTEQEFKDMCAAAEKYGVKVIVDAVINHTTSDYAAISDEIKRIPNWTHGNTQIKNWSDRWDVTQNSLLGLYDWNTQNTEVQTYLKRFLERALNDGADGFRYDAAKHIELPDDGNYGSRFWPNITNTSAEFQYGEILQDSASRDTAYANYMNVTASNYGHSIRSALKNRNLSVSNISHYASDVSADKLVTWVESHDTYANDEEESTWMSDDDIRLGWAVIGSRSGSTPLFFSRPEGGGNGVRFPGKSQIGDRGSALFKDQAITAVNQFHNVMAGQPEELSNPNGNNQIFMNQRGSKGVVLANAGSSSVTVNTSTKLPDGRYDNRAGAGSFQVANGKLTGTINARSAAVLYSDDIGNAPHVFLENYQTGAVHSFNDQLTVTLRANAKTAKAVYQINNGQQTAFKDGDRLTIGKGDPIGTTYNIRLTGTNGEGAERTQEYTFVKKDQAQ

Shinji Takenaka et al. (2015) — Characterization of the native form and the carboxy-terminally truncated halotolerant form of α-amylases from Bacillus subtilis strain FP-133

Journal of Basic Microbiology · doi:10.1002/jobm.201400813 ↗ · Activity - Classical

11 measurements ▼

Database ID

UniProt: A0A0P0YKS0 ↗

Sequence Annotation

Explicit - Provided (45 first residues and 99 last residues from the UniProt sequence absent from the mature protein)

Protein Source

Recombinant, host bacterium Bacillus subtilis RIK1285

Experimental Data (11 measurements)

11 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100.0	94.9	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Dimethylformamide (DMF)	25%	100.0	13.8	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Methanol	25%	100.0	97.0	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Acetonitrile	25%	100.0	87.8	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Ethanol	25%	100.0	88.7	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Acetone	25%	100.0	115.3	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Isopropanol	25%	100.0	102.2	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	1-Butanol	25%	100.0	104.3	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Chloroform	25%	100.0	0.1	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Benzene	25%	100.0	106.0	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Toluene	25%	100.0	113.0	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100.0	94.9	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Dimethylformamide (DMF)	25%	100.0	13.8	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Methanol	25%	100.0	97.0	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Acetonitrile	25%	100.0	87.8	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Ethanol	25%	100.0	88.7	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Acetone	25%	100.0	115.3	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Isopropanol	25%	100.0	102.2	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	1-Butanol	25%	100.0	104.3	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Chloroform	25%	100.0	0.1	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Benzene	25%	100.0	106.0	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Toluene	25%	100.0	113.0	%	50 mM sodium–potassium phosphate buffer	6.8	30°C	5 mg/mL Starch	Reducing sugars	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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