Cysteine synthase B (gene CysM) from Escherichia coli K12
Enzyme Description
Sequence
MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRAALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAAMRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQQTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPTEYLPGIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPDAVVVAIICDRGDRYLSTGVFGEEHFSQGAGI
Takashi Nakamura et al. (2015)
β Thermostability and reactivity in organic solvent of O -phospho-L-serine sulfhydrylase from hyperthermophilic archaeon Aeropyrum pernix K1
Bioscience, Biotechnology, and Biochemistry
Β· doi:10.1080/09168451.2015.1020753 β
Β· Activity - Classical
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Database ID
UniProt: P16703 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3) pLysS
Experimental Data (12 measurements)
12 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 5% | 100 | 82 | % | 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay | 6.7 | 37Β°C | 2.5 mM O-acetyl-L-serine, 2.5 mM Sodium sulfide | L-cysteine , Phosphate | 0.2 mM PLP | β | Classical aqueous control (in %) | Disagreement betweent text and figure for assay temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 10% | 100 | 82 | % | 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay | 6.7 | 37Β°C | 2.5 mM O-acetyl-L-serine, 2.5 mM Sodium sulfide | L-cysteine , Phosphate | 0.2 mM PLP | β | Classical aqueous control (in %) | Disagreement betweent text and figure for assay temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 20% | 100 | 92 | % | 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay | 6.7 | 37Β°C | 2.5 mM O-acetyl-L-serine, 2.5 mM Sodium sulfide | L-cysteine , Phosphate | 0.2 mM PLP | β | Classical aqueous control (in %) | Disagreement betweent text and figure for assay temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 30% | 100 | 54 | % | 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay | 6.7 | 37Β°C | 2.5 mM O-acetyl-L-serine, 2.5 mM Sodium sulfide | L-cysteine , Phosphate | 0.2 mM PLP | β | Classical aqueous control (in %) | Disagreement betweent text and figure for assay temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 40% | 100 | 17 | % | 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay | 6.7 | 37Β°C | 2.5 mM O-acetyl-L-serine, 2.5 mM Sodium sulfide | L-cysteine , Phosphate | 0.2 mM PLP | β | Classical aqueous control (in %) | Disagreement betweent text and figure for assay temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 50% | 100 | 2 | % | 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay | 6.7 | 37Β°C | 2.5 mM O-acetyl-L-serine, 2.5 mM Sodium sulfide | L-cysteine , Phosphate | 0.2 mM PLP | β | Classical aqueous control (in %) | Disagreement betweent text and figure for assay temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent | 1,4-Dioxane | 5% | 100 | 96 | % | 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay | 6.7 | 37Β°C | 2.5 mM O-acetyl-L-serine, 2.5 mM Sodium sulfide | L-cysteine , Phosphate | 0.2 mM PLP | β | Classical aqueous control (in %) | Disagreement betweent text and figure for assay temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent | 1,4-Dioxane | 10% | 100 | 69 | % | 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay | 6.7 | 37Β°C | 2.5 mM O-acetyl-L-serine, 2.5 mM Sodium sulfide | L-cysteine , Phosphate | 0.2 mM PLP | β | Classical aqueous control (in %) | Disagreement betweent text and figure for assay temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent | 1,4-Dioxane | 20% | 100 | 52 | % | 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay | 6.7 | 37Β°C | 2.5 mM O-acetyl-L-serine, 2.5 mM Sodium sulfide | L-cysteine , Phosphate | 0.2 mM PLP | β | Classical aqueous control (in %) | Disagreement betweent text and figure for assay temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent | 1,4-Dioxane | 30% | 100 | 13 | % | 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay | 6.7 | 37Β°C | 2.5 mM O-acetyl-L-serine, 2.5 mM Sodium sulfide | L-cysteine , Phosphate | 0.2 mM PLP | β | Classical aqueous control (in %) | Disagreement betweent text and figure for assay temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent | 1,4-Dioxane | 40% | 100 | 2 | % | 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay | 6.7 | 37Β°C | 2.5 mM O-acetyl-L-serine, 2.5 mM Sodium sulfide | L-cysteine , Phosphate | 0.2 mM PLP | β | Classical aqueous control (in %) | Disagreement betweent text and figure for assay temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent | 1,4-Dioxane | 50% | 100 | 1 | % | 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay | 6.7 | 37Β°C | 2.5 mM O-acetyl-L-serine, 2.5 mM Sodium sulfide | L-cysteine , Phosphate | 0.2 mM PLP | β | Classical aqueous control (in %) | Disagreement betweent text and figure for assay temperature |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.