O-phospho-l-serine sulfhydrylase from Aeropyrum pernix K1

Enzyme Description

Species

Aeropyrum pernix K1 ↗

Extremophile

Yes "hyperthermophilic" organism

EC Number

2.5.1.65 ↗

Sequence

Length: 389 amino acids

MALADISGYLDVLDSVRGFSYLENAREVLRSGEARCLGNPRSEPEYVKALYVIGASRIPVGDGCSHTLEELGVFDISVPGEMVFPSPLDFFERGKPTPLVRSRLQLPNGVRVWLKLEWYNPFSLSVKDRPAVEIISRLSRRVEKGSLVADATSSNFGVALSAVARLYGYRARVYLPGAAEEFGKLLPRLLGAQVIVDPEAPSTVHLLPRVMKDSKNEGFVHVNQFYNDANFEAHMRGTAREIFVQSRRGGLALRGVAGSLGTSGHMSAAAFYLQSVDPSIRAVLVQPAQGDSIPGIRRVETGMLWINMLDISYTLAEVTLEEAMEAVVEVARSDGLVIGPSGGAAVKALAKKAAEGDLEPGDYVVVVPDTGFKYLSLVQNALEGAGDSV

Takashi Nakamura et al. (2015) — Thermostability and reactivity in organic solvent of O -phospho-L-serine sulfhydrylase from hyperthermophilic archaeon Aeropyrum pernix K1

Bioscience, Biotechnology, and Biochemistry · doi:10.1080/09168451.2015.1020753 ↗ · Activity - Classical

12 measurements ▼

Database ID

UniProt: Q9YBL2 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3) pLysS

Experimental Data (12 measurements)

12 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	Dimethylformamide (DMF)	5%	100	170	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine , Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	183	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine , Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	255	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine , Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100	297	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine , Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	Dimethylformamide (DMF)	40%	100	280	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine , Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	Dimethylformamide (DMF)	50%	100	4	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine , Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	1,4-Dioxane	5%	100	182	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine , Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	1,4-Dioxane	10%	100	290	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine , Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	1,4-Dioxane	20%	100	459	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine , Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	1,4-Dioxane	30%	100	637	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine , Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	1,4-Dioxane	40%	100	89	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine , Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	1,4-Dioxane	50%	100	1	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine , Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	Dimethylformamide (DMF)	5%	100	170	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine, Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	183	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine, Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	255	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine, Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100	297	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine, Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	Dimethylformamide (DMF)	40%	100	280	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine, Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	Dimethylformamide (DMF)	50%	100	4	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine, Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	1,4-Dioxane	5%	100	182	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine, Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	1,4-Dioxane	10%	100	290	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine, Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	1,4-Dioxane	20%	100	459	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine, Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	1,4-Dioxane	30%	100	637	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine, Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	1,4-Dioxane	40%	100	89	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine, Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, acid ninhydrin reagent and cysteine reaction product absorbance measurement 560 nm) in the presence of organic solvent	1,4-Dioxane	50%	100	1	%	50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT assay	7.5	80°C	2.5 mM O-phospho-L-serine, 2.5 mM Sodium sulfide	L-cysteine, Phosphate	0.2 mM PLP	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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