Endoglucanase Cel45A from Neurospora crassa OR74A
Enzyme Description
Sequence
MRSSTILQTGLVAALPFAVQAASGSGQSTRYWDCCKPSCSWSGKAPVNRPVLACDANNNPLSDASVKSGCDGGSAYTCANNSPWAVNDQLSYGFAATKLSGGTESSWCCACYALTFTSGPVAGKTLVVQSTSTGGDLGSNHFDINMPGGGVGLFDGCKRQFGGLPGAQYGGISSRSQCDSFPAALKPGCQWRFDWFQNADNPNFTFKQVQCPSELTSRTGCKRNDDSQFPVFTPPSGGGSNPSTPTTPPSSGGGSGCTADKYAQCGGSGWSGCTNCPSGSTCKTINDYYHQCA
Marco Antonio Seiki Kadowaki et al. (2015)
β Functional Characterization and Low-Resolution Structure of an Endoglucanase Cel45A from the Filamentous Fungus Neurospora crassa OR74A: Thermostable Enzyme with High Activity Toward Lichenan and Ξ²-Glucan
Molecular Biotechnology
Β· doi:10.1007/s12033-015-9851-8 β
Β· Activity - Classical
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Database ID
UniProt: Q872Q1 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host yeast Aspergillus nidulans A773
Experimental Data (4 measurements)
4 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent | Acetone | 20% | 100.0 | 122.0 | % | ? | ? | 60Β°C | 0.5% (w/v) Ξ²-Glucan | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent | Isopropanol | 20% | 100.0 | 118.8 | % | ? | ? | 60Β°C | 0.5% (w/v) Ξ²-Glucan | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent | Methanol | 20% | 100.0 | 103.4 | % | ? | ? | 60Β°C | 0.5% (w/v) Ξ²-Glucan | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent | Ethanol | 20% | 100.0 | 119.6 | % | ? | ? | 60Β°C | 0.5% (w/v) Ξ²-Glucan | Reducing sugars | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.