Glucoside hydrolase (gene egl01) from a forest soil metagenomic library
Enzyme Description
Sequence
MSYMKRSISVFIACFMVAALGISGIIAPKAAAASKTPVAVNGQLTLKGTQLVNQNGKAVQLKGISSHGLQWCGDYVNKDSLKWLRDDWGINVFRAAMYTAEGGYIDNPSVKNKVKEAVEAAKELGIYVIIDWHILSDGNPNQNKAKAKEFFNEMSRLYGKTPNVIFEIANEPNGDVNWNRDIKPYAEEILSVIRKNSPKNIVIVGTGTWSQDVNDAADNQLKDGNVMYALHFYAGTHGQSLRDKADYALSKGAPIFVTEWGTSDASGNGGVYLDQSREWLKYLDSKKISWVNWNLSDKQESSAALNPGASKNGGWSQSDLSPSGKFVRDNIRSGSNGSSGDSGSNSKGSDQKDQKKDQDKPGQDSGAAANTIAVQYRAGDNNVNGNQIRPQLNIKNNSKKTVSLNRITVRYWYKTNRKGQNFDCDYAQIGCSKITHKFVQLKKAVNGADTYLEVGFKNGTLAPGADTGEIQIRLHNDGWSNYAQSGDYSFFNSNTFKNTKKITLYENGKLIWGTEPK
Mei Hua et al. (2015)
β Direct detection, cloning and characterization of a glucoside hydrolase from forest soil
Biotechnology Letters
Β· doi:10.1007/s10529-015-1777-5 β
Β· Activity - Classical
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Database ID
UniProt: A0A0A7EZH7 β
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (10 measurements)
10 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Methanol | 1% | 100 | 104 | % | 200 mM Tris-HCl | 7.6 | 50Β°C | 1% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Methanol | 10% | 100 | 96 | % | 200 mM Tris-HCl | 7.6 | 50Β°C | 1% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Ethanol | 1% | 100 | 103 | % | 200 mM Tris-HCl | 7.6 | 50Β°C | 1% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Ethanol | 10% | 100 | 93 | % | 200 mM Tris-HCl | 7.6 | 50Β°C | 1% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Acetonitrile | 1% | 100 | 100 | % | 200 mM Tris-HCl | 7.6 | 50Β°C | 1% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Acetonitrile | 10% | 100 | 94 | % | 200 mM Tris-HCl | 7.6 | 50Β°C | 1% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Isopropanol | 1% | 100 | 102 | % | 200 mM Tris-HCl | 7.6 | 50Β°C | 1% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Isopropanol | 10% | 100 | 93 | % | 200 mM Tris-HCl | 7.6 | 50Β°C | 1% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Glycerol | 1% | 100 | 104 | % | 200 mM Tris-HCl | 7.6 | 50Β°C | 1% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Glycerol | 10% | 100 | 100 | % | 200 mM Tris-HCl | 7.6 | 50Β°C | 1% (w/v) CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.