Laccase/phenol oxidase from Pleurotus ostreatus

Enzyme Description

Species

Extremophile

EC Number

1.10.3.2 ↗

Sequence

Length: 581 amino acids

AQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGAMFPGARILATLTLALHLLHGAHAAIGPAGNMYIVNEDVSPDGFARSAVVARSVPATDPTPATASIPGVLVQGNKGDNFQLNVVNQLSDTTMLKTTSIHWHGFFQAGSSWADGPAFVTQCPVASGDSFLYNFNVPDQAGTFWYHSHLSTQYCDGLRGPFVVYDPSDPHLSLYDIDNADTVITLEDWYHIVAPQNAAIPTPDSTLINGKGRYAGGPTSPLAIINVESNKRYRFRLVSMSCDPNFTFSIDGHSLLVIEADAVNIVPITVDSIQIFAGQRYSFVLTANQAVDNYWIRANPNLGSTGFVGGINSAILRYAGATEDDPTTTSSTSTPLLETNLVPLENPGAPGPPVPGGADININLAMAFDFTTFELTINGVPFLPPTAPVLLQILSGASTAASLLPSGSIYELEANKVVEISMPALAVGGPHPFHLHGHTFDVIRSAGSTTYNFDTPARRDVVNTGTGANDNVTIRFVTDNPGPWFLHCHIDWHLEIGLAVVFAEDVTSISAPPAAWDDLCPIYNALSDNDKGGIVPS

G. Palmeiri et al. (1993) — Stability and activity of a phenol oxidase from the ligninolytic fungus Pleurotus ostreatus

Applied Microbiology and Biotechnology · doi:10.1007/BF00205066 ↗ · Stability - Incubation

6 measurements ▼

Database ID

UniProt: Q12739 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, fungus Pleurotus ostreatus

Experimental Data (6 measurements)

6 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (60 minutes at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100.0	70.4	%	Incubation: ?, Assay: 100 mM Na-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	2 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase, uncertainty about assay conditions. Here laccase activity measured, and protein classified as such in UniProt, while authors call it "phenol oxidase"
Stability - Incubation	Activity after incubation (60 minutes at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	77.1	%	Incubation: ?, Assay: 100 mM Na-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	2 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase, uncertainty about assay conditions. Here laccase activity measured, and protein classified as such in UniProt, while authors call it "phenol oxidase"
Stability - Incubation	Activity after incubation (60 minutes at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	50%	100.0	5.6	%	Incubation: ?, Assay: 100 mM Na-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	2 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase, uncertainty about assay conditions. Here laccase activity measured, and protein classified as such in UniProt, while authors call it "phenol oxidase"
Stability - Incubation	Activity after incubation (60 minutes at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.0	8.5	%	Incubation: ?, Assay: 100 mM Na-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	2 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase, uncertainty about assay conditions. Here laccase activity measured, and protein classified as such in UniProt, while authors call it "phenol oxidase"
Stability - Incubation	Activity after incubation (60 minutes at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100.0	13.5	%	Incubation: ?, Assay: 100 mM Na-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	2 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase, uncertainty about assay conditions. Here laccase activity measured, and protein classified as such in UniProt, while authors call it "phenol oxidase"
Stability - Incubation	Activity after incubation (60 minutes at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethyl Acetate	50%	100.0	100.0	%	Incubation: ?, Assay: 100 mM Na-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	2 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase, uncertainty about assay conditions. Here laccase activity measured, and protein classified as such in UniProt, while authors call it "phenol oxidase"

Stability - Incubation	Activity after incubation (60 minutes at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100.0	70.4	%	Incubation: ?, Assay: 100 mM Na-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	2 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase, uncertainty about assay conditions. Here laccase activity measured, and protein classified as such in UniProt, while authors call it "phenol oxidase"
Stability - Incubation	Activity after incubation (60 minutes at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	77.1	%	Incubation: ?, Assay: 100 mM Na-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	2 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase, uncertainty about assay conditions. Here laccase activity measured, and protein classified as such in UniProt, while authors call it "phenol oxidase"
Stability - Incubation	Activity after incubation (60 minutes at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1,4-Dioxane	50%	100.0	5.6	%	Incubation: ?, Assay: 100 mM Na-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	2 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase, uncertainty about assay conditions. Here laccase activity measured, and protein classified as such in UniProt, while authors call it "phenol oxidase"
Stability - Incubation	Activity after incubation (60 minutes at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.0	8.5	%	Incubation: ?, Assay: 100 mM Na-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	2 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase, uncertainty about assay conditions. Here laccase activity measured, and protein classified as such in UniProt, while authors call it "phenol oxidase"
Stability - Incubation	Activity after incubation (60 minutes at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100.0	13.5	%	Incubation: ?, Assay: 100 mM Na-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	2 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase, uncertainty about assay conditions. Here laccase activity measured, and protein classified as such in UniProt, while authors call it "phenol oxidase"
Stability - Incubation	Activity after incubation (60 minutes at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethyl Acetate	50%	100.0	100.0	%	Incubation: ?, Assay: 100 mM Na-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	2 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really was in aqueous phase, uncertainty about assay conditions. Here laccase activity measured, and protein classified as such in UniProt, while authors call it "phenol oxidase"

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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