Lipase (gene mas1) from Streptomyces sp. strain W007
Enzyme Description
Sequence
MLPWIRAARVPRTRSLLAALLLALTALVAPAATATAATPAAEATSRGWNDYSCKPSAAHPRPVVLVHGTFGNSIDNWLVLAPYLVNRGYCVFSLDYGQLPGVPFFHGLGPIDKSAEQLDVFVDKVLDATGAPKADLVGHSQGGMMPNYYLKFLGGADKVNALVGIAPDNHGTTLLGLTKLLPFFPGVEKFISDNTPGLADQVAGSPFITKLTAGGDTVPGVRYTVIATKYDQVVTPYRTQYLDGPNVRNVLLQDLCPVDLSEHVAIGTIDRIAFHEVANALDPARATPTTCASVIG
Dongjuan Yuan et al. (2015)
β Screening and characterization of a thermostable lipase from marine Streptomyces sp. strain W007
Biotechnology and Applied Biochemistry
Β· doi:10.1002/bab.1338 β
Β· Stability - Incubation
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Database ID
UniParc: UPI000241A407 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host yeast Pichia pastoris X33
Experimental Data (4 measurements)
4 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (2 hours at 4Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Methanol | 1% | 100.0 | 88.92 | % | Incubation: 0.1 M phosphate buffer, Assay: 0.1 M phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 4Β°C, Assay: 40Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 4Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Ethanol | 1% | 100.0 | 92.8 | % | Incubation: 0.1 M phosphate buffer, Assay: 0.1 M phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 4Β°C, Assay: 40Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 4Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Isopropanol | 1% | 100.0 | 97.09 | % | Incubation: 0.1 M phosphate buffer, Assay: 0.1 M phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 4Β°C, Assay: 40Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 4Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetone | 1% | 100.0 | 95.01 | % | Incubation: 0.1 M phosphate buffer, Assay: 0.1 M phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 4Β°C, Assay: 40Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.