Xylanase (gene XAn11) from Aspergillus niger US368
Enzyme Description
Sequence
MKVTAAFASLLLTAFAAPAPEPVLVSRSAGINYVQNYNGNLGDFTYDESAGTFSMYWEDGVSSDFVVGLGWTTGSSKSITYSAQYSASSSSSYLAVYGWVNSPQAEYYIVEDYGDYNPCSSATSLGTVYSDGSTYQVCTDTRTNAPSITGTSTFTQYFSVRESTRTSGTVTVANHFNFWAQHGFGNSNFNYQVMAVEAWNGAGSASVTIST
AΓ―da Hmida-Sayari et al. (2012)
β Biochemical characterization, cloning and molecular modeling of a detergent and organic solvent-stable family 11 xylanase from the newly isolated Aspergillus niger US368 strain
Process Biochemistry
Β· doi:10.1016/j.procbio.2012.06.010 β
Β· Stability - Incubation
▼
Database ID
UniProt: E3UN71 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Purified, bacterium Aspergillus niger US368
Experimental Data (7 measurements)
7 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Ethanol | 1% | 100.0 | 126.0 | % | Incubation: ?, Assay: 0.13 M sodium acetate buffer | Incubation: ?, Assay: 5 | Incubation: 40Β°C, Assay: 55Β°C | 0.33% Soluble birchwood xylan | Reducing sugars | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about control and whether assay was with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Isopropanol | 1% | 100.0 | 124.0 | % | Incubation: ?, Assay: 0.13 M sodium acetate buffer | Incubation: ?, Assay: 5 | Incubation: 40Β°C, Assay: 55Β°C | 0.33% Soluble birchwood xylan | Reducing sugars | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about control and whether assay was with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | 1-Butanol | 1% | 100.0 | 123.0 | % | Incubation: ?, Assay: 0.13 M sodium acetate buffer | Incubation: ?, Assay: 5 | Incubation: 40Β°C, Assay: 55Β°C | 0.33% Soluble birchwood xylan | Reducing sugars | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about control and whether assay was with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Methanol | 1% | 100.0 | 117.2 | % | Incubation: ?, Assay: 0.13 M sodium acetate buffer | Incubation: ?, Assay: 5 | Incubation: 40Β°C, Assay: 55Β°C | 0.33% Soluble birchwood xylan | Reducing sugars | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about control and whether assay was with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Glycerol | 1% | 100.0 | 104.25 | % | Incubation: ?, Assay: 0.13 M sodium acetate buffer | Incubation: ?, Assay: 5 | Incubation: 40Β°C, Assay: 55Β°C | 0.33% Soluble birchwood xylan | Reducing sugars | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about control and whether assay was with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Acetone | 1% | 100.0 | 99.32 | % | Incubation: ?, Assay: 0.13 M sodium acetate buffer | Incubation: ?, Assay: 5 | Incubation: 40Β°C, Assay: 55Β°C | 0.33% Soluble birchwood xylan | Reducing sugars | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about control and whether assay was with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Chloroform | 1% | 100.0 | 97.43 | % | Incubation: ?, Assay: 0.13 M sodium acetate buffer | Incubation: ?, Assay: 5 | Incubation: 40Β°C, Assay: 55Β°C | 0.33% Soluble birchwood xylan | Reducing sugars | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about control and whether assay was with or without organic solvent |
Visualization : Stability β Incubation
AΓ―da Hmida-Sayari et al. (2012)
One bar per measurement. Colour = solvent, shade = solvent volume.
Fatma Elgharbi et al. (2015)
β Expression of A. niger US368 xylanase in E. coli: Purification, characterization and copper activation
International Journal of Biological Macromolecules
Β· doi:10.1016/j.ijbiomac.2014.12.005 β
Β· Stability - Incubation
▼
Database ID
UniProt: E3UN71 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3) pLysS
Experimental Data (7 measurements)
7 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Ethanol | 1% | 100.0 | 130.2 | % | Incubation: ?, Assay: 500 mM citrate buffer | Incubation: ?, Assay: 5 | Incubation: 40Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Isopropanol | 1% | 100.0 | 122.64 | % | Incubation: ?, Assay: 500 mM citrate buffer | Incubation: ?, Assay: 5 | Incubation: 40Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | 1-Butanol | 1% | 100.0 | 112.03 | % | Incubation: ?, Assay: 500 mM citrate buffer | Incubation: ?, Assay: 5 | Incubation: 40Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Methanol | 1% | 100.0 | 104.8 | % | Incubation: ?, Assay: 500 mM citrate buffer | Incubation: ?, Assay: 5 | Incubation: 40Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Glycerol | 1% | 100.0 | 102.12 | % | Incubation: ?, Assay: 500 mM citrate buffer | Incubation: ?, Assay: 5 | Incubation: 40Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Acetone | 1% | 100.0 | 98.36 | % | Incubation: ?, Assay: 500 mM citrate buffer | Incubation: ?, Assay: 5 | Incubation: 40Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase, uncertainty about control |
| Stability - Incubation | Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Chloroform | 1% | 100.0 | 95.45 | % | Incubation: ?, Assay: 500 mM citrate buffer | Incubation: ?, Assay: 5 | Incubation: 40Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase, uncertainty about control |
Visualization : Stability β Incubation
Fatma Elgharbi et al. (2015)
One bar per measurement. Colour = solvent, shade = solvent volume.