Haloperoxidase BPO-A1 from Streptomyces aureofaciens

Enzyme Description

Species

Extremophile

EC Number

1.11.1.- ↗

Sequence

Length: 275 amino acids

MPICTTRDGVEIFYKDWGQGRPVVFIHGWPLNGDAWQDQLKAVVDAGYRGIAHDRRGHGHSTPVWDGYDFDTFADDLNDLLTDLDLRDVTLVAHSMGGGELARYVGRHGTGRLRSAVLLSAIPPVMIKSDKNPDGVPDEVFDALKNGVLTERSQFWKDTAEGFFSANRPGNKVTQGNKDAFWYMAMAQTIEGGVRCVDAFGYTDFTEDLKKFDIPTLVVHGDDDQVVPIDATGRKSAQIIPNAELKVYEGSSHGIAMVPGDKEKFNRDLLEFLNK

Ryosuke Yamada et al. (2014) — Improvement of the stability and activity of the BPO-A1 haloperoxidase from Streptomyces aureofaciens by directed evolution

Journal of Biotechnology · doi:10.1016/j.jbiotec.2014.10.030 ↗ · Stability - Incubation

27 measurements ▼

Database ID

UniProt: P33912 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli Rosetta2 (DE3)

Experimental Data (27 measurements)

27 measurements — page 1 of 2

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
WT	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Methanol	40%	93	—	89	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	20%	93	—	78	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
WT	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethylformamide (DMF)	40%	93	—	87	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
WT	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Ethanol	40%	93	—	69	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	30%	93	—	58	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	10%	93	—	96	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
WT	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	93	—	107	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	40%	93	—	26	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	50%	93	—	6	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
G106S/V148I	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Methanol	40%	96	89	79	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
R114H/N146H	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	50%	100	6	18	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
R114H/N146H	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Ethanol	40%	100	69	92	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
G106S/V148I	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Ethanol	40%	96	69	18	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
R114H/N146H	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	100	107	111	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
G106S/V148I	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	96	107	60	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
R114H/N146H	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethylformamide (DMF)	40%	100	87	110	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
G106S/V148I	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethylformamide (DMF)	40%	96	87	67	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
R114H/N146H	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Methanol	40%	100	89	104	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
G106S/V148I	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	50%	96	6	11	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
G106S/V148I	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	40%	96	26	15	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase

WT	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Methanol	40%	93	—	89	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	20%	93	—	78	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
WT	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethylformamide (DMF)	40%	93	—	87	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
WT	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Ethanol	40%	93	—	69	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	30%	93	—	58	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	10%	93	—	96	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
WT	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	93	—	107	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	40%	93	—	26	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	50%	93	—	6	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
G106S/V148I	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Methanol	40%	96	89	79	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
R114H/N146H	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	50%	100	6	18	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
R114H/N146H	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Ethanol	40%	100	69	92	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
G106S/V148I	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Ethanol	40%	96	69	18	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
R114H/N146H	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	100	107	111	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
G106S/V148I	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	96	107	60	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
R114H/N146H	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethylformamide (DMF)	40%	100	87	110	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
G106S/V148I	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethylformamide (DMF)	40%	96	87	67	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
R114H/N146H	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Methanol	40%	100	89	104	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
G106S/V148I	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	50%	96	6	11	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
G106S/V148I	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	40%	96	26	15	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
R114H/N146H	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	40%	100	26	55	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
G106S/V148I	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	30%	96	58	60	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
R114H/N146H	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	30%	100	58	71	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
G106S/V148I	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	20%	96	78	86	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
R114H/N146H	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	20%	100	78	79	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
G106S/V148I	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	10%	96	96	98	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
R114H/N146H	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (colorimetric assay, chlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Butanol	10%	100	96	94	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN3, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	10 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase

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Mutations in this dataset (3) — Ryosuke Yamada et al. (2014)

WT G106S/V148I R114H/N146H

Visualization : Stability — Incubation

Ryosuke Yamada et al. (2014)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Ryosuke Yamada et al. (2015) — Random mutagenesis and selection of organic solvent-stable haloperoxidase from Streptomyces aureofaciens

Biotechnology Progress · doi:10.1002/btpr.2117 ↗ · Stability - Incubation

34 measurements ▼

Database ID

UniProt: P33912 ↗

Sequence Annotation

Inferred - from protein name (corresponding mutated residues numbering)

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (34 measurements)

34 measurements — page 1 of 2

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	93.0	—	25.9	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	86.0	—	107.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Ethanol	40%	86.0	—	69.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	10%	93.0	—	97.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Methanol	40%	86.0	—	89.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethylformamide (DMF)	40%	86.0	—	87.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	50%	93.0	—	5.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	93.0	—	26.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	30%	93.0	—	58.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	20%	93.0	—	78.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P123L/P241A	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	104.0	25.9	46.5	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P123L	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	—	25.9	81.7	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
H53Y/G162R	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Methanol	40%	100.0	89.0	66.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
H53Y/G162R	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethylformamide (DMF)	40%	100.0	87.0	90.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P123L/P241A	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethylformamide (DMF)	40%	97.0	87.0	104.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P241A	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	—	25.9	6.92	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
H53Y/G162R	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	109.0	25.9	18.6	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
H53Y/G162R	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	100.0	107.0	72.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
H53Y	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	—	25.9	33.4	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P123L/P241A	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	97.0	107.0	111.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O , HOBr	—	—	Water-incubated control (in %), assay in aqueous phase

WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	93.0	—	25.9	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	86.0	—	107.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Ethanol	40%	86.0	—	69.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	10%	93.0	—	97.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Methanol	40%	86.0	—	89.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethylformamide (DMF)	40%	86.0	—	87.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	50%	93.0	—	5.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	93.0	—	26.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	30%	93.0	—	58.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
WT	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	20%	93.0	—	78.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P123L/P241A	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	104.0	25.9	46.5	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P123L	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	—	25.9	81.7	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
H53Y/G162R	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Methanol	40%	100.0	89.0	66.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
H53Y/G162R	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethylformamide (DMF)	40%	100.0	87.0	90.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P123L/P241A	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethylformamide (DMF)	40%	97.0	87.0	104.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P241A	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	—	25.9	6.92	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
H53Y/G162R	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	109.0	25.9	18.6	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
H53Y/G162R	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	100.0	107.0	72.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
H53Y	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	—	25.9	33.4	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P123L/P241A	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	97.0	107.0	111.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
G162R	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	—	25.9	17.4	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
H53Y/G162R	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Ethanol	40%	100.0	69.0	2.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P123L/P241A	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Ethanol	40%	97.0	69.0	98.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P123L/P241A	Stability - Incubation	Activity after incubation (48 hours at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	Methanol	40%	97.0	89.0	101.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
H53Y/G162R	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	50%	109.0	5.0	6.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P123L/P241A	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	50%	104.0	5.0	4.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
H53Y/G162R	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	109.0	26.0	21.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P123L/P241A	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	40%	104.0	26.0	46.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
H53Y/G162R	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	30%	109.0	58.0	52.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P123L/P241A	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	30%	104.0	58.0	79.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
H53Y/G162R	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	20%	109.0	78.0	79.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P123L/P241A	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	20%	104.0	78.0	87.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
H53Y/G162R	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	10%	109.0	97.0	98.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase
P123L/P241A	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in aqueous phase	1-Propanol	10%	104.0	97.0	95.0	%	Incubation: ?, Assay: 1 M sodium acetate buffer, 10 mM NaN₃, 50 µM Chlorodimedone	Incubation: ?, Assay: 5.5	Incubation: 30°C, Assay: 25°C	9 mM H₂O₂ (Hydrogen Peroxide), 100 mM Sodium bromide	H2O, HOBr	—	—	Water-incubated control (in %), assay in aqueous phase

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Mutations in this dataset (7) — Ryosuke Yamada et al. (2015)

WT H53Y H53Y/G162R P123L P123L/P241A G162R P241A

Visualization : Stability — Incubation

Ryosuke Yamada et al. (2015)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Structure

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Haloperoxidase BPO-A1 from Streptomyces aureofaciens

Enzyme Description

Sequence

Experimental Data (27 measurements)

Visualization : Stability — Incubation

Mutation Effect

Experimental Data (34 measurements)

Visualization : Stability — Incubation

Mutation Effect

Structure

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