Lipase 3646 from Cohnella sp. A01

Enzyme Description

Species
Cohnella sp. A01 β†—
Extremophile
Yes "thermophilic" organism
EC Number
3.1.1.3 β†—

Sequence

Length: 268 amino acids
MRKGGYDPAWAIFLAAVCSQTYEQYNNKEGQFVVPLGYRTAFTVDATSFDRKRERFGFILESDRDIVVAFRGTSSTADWVSDALAYQIRYPYRDKAGQTHQGFTHIYRSARARIVSALTSLPPDKPVYVAGHSLGGALAVLCALDLATLDSRRLLAAYTFGAPRTGDPGFARAFNAAVRKSFRIANPYDAVAQLPPFILRMPGSKKTYYYSHVRGAVVLPFQNGSPAANHLIGSYFAALAARDPAYAERLEADNPGFCPVRRTGKNRG
Bahram pooreydy Golaki et al. (2015) β€” Cloning, expression, purification, and characterization of lipase 3646 from thermophilic indigenous Cohnella sp. A01
Protein Expression and Purification  Β· doi:10.1016/j.pep.2014.10.002 β†—  Β· Stability - Incubation
9 measurements ▼
Database ID
UniProt: K7W8S6 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (9 measurements)

9 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 30% 100.0 58.0 % Incubation: ?, Assay: 50 mM Tris-base buffer, 1% acetonitrile, 4% Isopropanol Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 70Β°C 3 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 30% 100.0 94.3 % Incubation: ?, Assay: 50 mM Tris-base buffer, 1% acetonitrile, 4% Isopropanol Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 70Β°C 3 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 30% 100.0 92.1 % Incubation: ?, Assay: 50 mM Tris-base buffer, 1% acetonitrile, 4% Isopropanol Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 70Β°C 3 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 30% 100.0 88.4 % Incubation: ?, Assay: 50 mM Tris-base buffer, 1% acetonitrile, 4% Isopropanol Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 70Β°C 3 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexane 30% 100.0 94.0 % Incubation: ?, Assay: 50 mM Tris-base buffer, 1% acetonitrile, 4% Isopropanol Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 70Β°C 3 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Heptane 30% 100.0 93.5 % Incubation: ?, Assay: 50 mM Tris-base buffer, 1% acetonitrile, 4% Isopropanol Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 70Β°C 3 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Chloroform 30% 100.0 74.8 % Incubation: ?, Assay: 50 mM Tris-base buffer, 1% acetonitrile, 4% Isopropanol Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 70Β°C 3 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Diethyl Ether 30% 100.0 75.6 % Incubation: ?, Assay: 50 mM Tris-base buffer, 1% acetonitrile, 4% Isopropanol Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 70Β°C 3 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Glycerol 30% 100.0 110.3 % Incubation: ?, Assay: 50 mM Tris-base buffer, 1% acetonitrile, 4% Isopropanol Incubation: ?, Assay: 8.5 Incubation: Room temperature, Assay: 70Β°C 3 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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