Ξ±-amylase from Bacillus subtilis DR8806
Enzyme Description
Sequence
MFAKRFKTSLLPLFAGFLLLFHLVLAGPAAASAETANKSNELTAPSIKSGTILHAWNWSFNTLKHNMKDIHDAGYTAIQTSPINQVKEGNQGDKSMSNWYWLYQPTSYQIGNRYLGTEQEFKEMCAAAEEYGIKVIVDAVINHTTSDYAAISNEVKSIPNWTHGNTQIKNWSDRWDVTQNSLLGLYDWNTQNTQVQSYLKRFLDRALNDGADGFRFDAAKHIELPDDGSYGSQFWPNITNTSAEFQYGEILQDSASRDAAYANYMDVTASNYGHSIRSALKNRNLGVSNISHYASDVSADKLVTWVESHDTYANDDEESTWMSDDDIRLGWAVIASRSGSTPLFFSRPEGGGNGVRFPGKSQIGDRGSALFEDQAITAVNRFHNVMAGQPEELSNPNGNNQIFMNQRGSHGVVLANAGSSSVSINTATKLPDGRYDNKAGAGSFQVNDGKLTGTINARSVAVLYPDDIAKAPHVFLENYKTGVTHSFNDQLTITLRADANTTKAVYQINNGPETAFKDGDQFTIGKGDPFGKTYTIMLKGTNSDGVTRTEKYSFVKRDPASAKTIGYQNPNHWSQVNAYIYKHDGSRVIELTGSWPGKPMTKNADGIYTLTLPADTDTTNAKVIFNNGSAQVPGQNQPGFDYVLNGLYNDSGLSGSLPH
Shamsi Emtenani et al. (2015)
β Gene cloning and characterization of a thermostable organic-tolerant Ξ±-amylase from Bacillus subtilis DR8806
International Journal of Biological Macromolecules
Β· doi:10.1016/j.ijbiomac.2014.08.023 β
Β· Stability - Incubation
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Database ID
UniProt: C0KWE6 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (20 measurements)
20 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Acetone | 20% | 100 | 85 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Toluene | 20% | 100 | 98 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Diethyl Ether | 20% | 100 | 77 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Isoamyl Alcohol | 20% | 100 | 64 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | n-Heptane | 20% | 100 | 129 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | n-Hexane | 20% | 100 | 114 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Isopropanol | 20% | 100 | 105 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | 1-Butanol | 20% | 100 | 163 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Ethanol | 20% | 100 | 144 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Methanol | 20% | 100 | 105 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Acetone | 10% | 100 | 116 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Toluene | 10% | 100 | 139 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Diethyl Ether | 10% | 100 | 99 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Isoamyl Alcohol | 10% | 100 | 117 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | n-Heptane | 10% | 100 | 104 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | n-Hexane | 10% | 100 | 112 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Isopropanol | 10% | 100 | 77 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | 1-Butanol | 10% | 100 | 68 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Ethanol | 10% | 100 | 128 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Methanol | 10% | 100 | 113 | % | Incubation: 50 mM sodium acetate buffer, Assay: 50 mM sodium acetate buffer | Incubation: 5, Assay: 5 | Incubation: Room temperature, Assay: 70Β°C | 1% Soluble potato starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.