Ξ²-xylosidase (gene XsidB) from Geobacillus thermodenitrificans TSAS1
Enzyme Description
Extremophile
Yes
"extremely thermophilic" organism
EC Number
Sequence
MPNNLFFNAHHSPVGAFSSFTLGFPGKSGGLDLELARPPRQNVFIGVESLHESGLYHVLPFWETSGEDESKRYDIENPDPNPQKPNILIPFAKEEIQREFHVATDTWKAGDLTFTIYSPVKAVPDPETADEEALKLALVPAVIVEMKIDNTNGTRARRAFFGFEGTDPYTSMRRIDETCPQLRGVGQGRIVGIVSKDEDVRSALHFSMEDILTAQLEENWTFGLGKVGALIVDVPAGEKKTYQFAVCFYRGGYVTAGMDTSYFYTRFFNNIEEVGLYALEKAEVLKEQSFRSNELIEKEWLSDDQKFMMAHAIRSFITAIHSCLSMKESRFGVNEGEYRMMNTFDLTVDQLFFELKMNPWTVKNVLDLYVERYSYEDGVRFPGEETEYPGGISFTHDMGVANTFLRPHHSSYELYGLSGCFSHMTHEQLVNWVLCAAVYIEQTKDWAWRDKRLAILEQCLESMVRRDHPDPEKRNGIMGLDSTRTMGGAEITTYDSLDVSLGQARNNLYLAGKCWAAYVALEKLFHDVGKEELAALAGEQAEKCAATIVSYVTDDGYIPAVMGEGNDSKIIPAIEGLVFPYFTNCHEALNKDGRFGEYIQALNAHLRYVLREGICLFPDGGWKISSTSNNSWLSKIYLCQFIARHLLGWEWDEQGKRADAAHVAWLTHPTLSIWSWSDQIIAGEIKASKYYPRGVTS
Ira Jain et al. (2014)
β Applicability of recombinant Ξ²-xylosidase from the extremely thermophilic bacterium Geobacillus thermodenitrificans in synthesizing alkylxylosides
Bioresource Technology
Β· doi:10.1016/j.biortech.2014.07.113 β
Β· Activity - Classical
▼
Database ID
UniProt: V5N4G7 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (18 measurements)
18 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | 1-Butanol | 5% | 100.0 | 76.78 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Benzene | 10% | 100.0 | 81.51 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Chloroform | 10% | 100.0 | 86.24 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | 1-Butanol | 10% | 100.0 | 71.56 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Ethanol | 10% | 100.0 | 70.3 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Methanol | 10% | 100.0 | 71.88 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Acetone | 10% | 100.0 | 48.01 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Benzene | 5% | 100.0 | 82.8 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Chloroform | 5% | 100.0 | 88.46 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Acetone | 1% | 100.0 | 73.18 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Ethanol | 5% | 100.0 | 72.48 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Methanol | 5% | 100.0 | 77.28 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Acetone | 5% | 100.0 | 72.7 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Benzene | 1% | 100.0 | 83.74 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Chloroform | 1% | 100.0 | 88.68 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | 1-Butanol | 1% | 100.0 | 80.05 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Ethanol | 1% | 100.0 | 76.67 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Methanol | 1% | 100.0 | 87.95 | % | ? | ? | 70Β°C | 0.5 mM p-nitrophenyl Ξ²-D-xylopyranoside | D-Xylose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.