Cellulase (gene CS10) from a Hermetia illucens gut microflora metagenomic library
Enzyme Description
Species
Na (Hermetia illucens gut microflora metagenomic library)
Extremophile
No
EC Number
Sequence
MKKISIYLLGLILTLLSVFPVGCFSNNPIEGDKSDFVAINGKLSVQGTSLVNQAGEPVVLRGVSLGWHNWWPRFYNKSTVAWMKRDWNCNLIRAAIGVDPKKGYIDNPDFSIACLTNVVDAAIENNMYVIIDWHSHHIRLEEAKAFFTLAAEKYKDYPNIIYEIYNEPIDDSWEEVKAYSEEIIKTIRSIDKDNVILVGTPHWDQDIHLAADNPITGYTNIMYTLHFYAGTHKQELRDRADYALAKDLPIFVSECAGMNADGDGAINLEEWNTWKEWMSRHRISWAAWSIADKNETCSMIKSKKSPAYGWTENDLKEWGKIIRNTLQKENK
Chang-Muk Lee et al. (2014)
β Screening and Characterization of a Novel Cellulase Gene from the Gut Microflora of Hermetia illucens Using Metagenomic Library
Journal of Microbiology and Biotechnology
Β· doi:10.4014/jmb.1405.05001 β
Β· Stability - Incubation
▼
Database ID
UniParc: UPI00032B509B β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (16 measurements)
16 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 97 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 50Β°C | 0.5% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 62 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 50Β°C | 0.5% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Dimethylformamide (DMF) | 10% | 100 | 87 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 50Β°C | 0.5% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Dimethylformamide (DMF) | 20% | 100 | 56 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 50Β°C | 0.5% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Methanol | 10% | 100 | 91 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 50Β°C | 0.5% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Methanol | 20% | 100 | 61 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 50Β°C | 0.5% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Acetonitrile | 10% | 100 | 83 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 50Β°C | 0.5% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Acetonitrile | 20% | 100 | 43 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 50Β°C | 0.5% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Ethanol | 10% | 100 | 84 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 50Β°C | 0.5% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Ethanol | 20% | 100 | 52 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 50Β°C | 0.5% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Acetone | 10% | 100 | 78 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 50Β°C | 0.5% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Acetone | 20% | 100 | 44 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 50Β°C | 0.5% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Isopropanol | 10% | 100 | 63 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 50Β°C | 0.5% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Isopropanol | 20% | 100 | 35 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 50Β°C | 0.5% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | 1-Butanol | 10% | 100 | 4 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 50Β°C | 0.5% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Clear about assay |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | 1-Butanol | 20% | 100 | 0 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer | Incubation: 7, Assay: 7 | Incubation: 30Β°C, Assay: 50Β°C | 0.5% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Clear about assay |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.