Esterase from Paenibacillus sp. PBS-2

Enzyme Description

Species
Paenibacillus sp. PBS-2 β†—
Extremophile
No but"cold-adapted" enzyme
EC Number
3.1.1.1 β†—

Sequence

Length: 377 amino acids
MDFKPVASLIDRLTSWRIPWAEVLVMHHNEIVFQYRNGYENLEEKTKIRDGAIMNLYSMTKIMTCAAALQLVEKGDILLLDPLSQYLPEYGDMQVRKTLPNGEVYYEKATRPITVRDLFTMTAGFSYNVNAPSIKEAVERTNGALPTREFAKAFAKEPLMFEPGTHWNYSMCHDVLGALIEVVSGKRFGTYLQDEIIKPLGMHDTAFDLNEEQQSRLIPQYIFDEEQNKAVRMDGNGFRVGTELDSGGAGLLSTVNDYAKFLNALTGLGTSPEGVRILSPASVNLMRTDHLNDMTRPDFSWDHMYGYGYGLGVRTHISKAGSGSLSPLGEFGWSGAAGCMAIMDPDSGLTVMYAQHLLNSQEHYVQRRLRNAVYSCL
Young-Ok Kim et al. (2014) β€” A Novel Esterase from Paenibacillus sp. PBS-2 Is a New Member of the Ξ²-Lactamase Belonging to the Family VIII Lipases/Esterases
Journal of Microbiology and Biotechnology  Β· doi:10.4014/jmb.1405.05043 β†—  Β· Stability - Incubation
14 measurements ▼
Database ID
UniProt: A0A0B4LBA5 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (14 measurements)

14 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 15% 100 99 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: Room temerature, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 15% 100 102 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: Room temerature, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 15% 100 104 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: Room temerature, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 15% 100 103 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: Room temerature, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 15% 100 104 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: Room temerature, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase PEG-8000 15% 100 101 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: Room temerature, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 15% 100 103 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: Room temerature, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 30% 100 102 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: Room temerature, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 30% 100 104 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: Room temerature, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 30% 100 104 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: Room temerature, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 30% 100 110 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: Room temerature, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetonitrile 30% 100 62 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: Room temerature, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase PEG-8000 30% 100 103 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: Room temerature, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30% 100 106 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: Room temerature, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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