Laccase (gene CgL1) from Corynebacterium glutamicum ATCC 13032

Enzyme Description

Species
Corynebacterium glutamicum ATCC 13032 β†—
Extremophile
No
EC Number
1.10.3.2 β†—

Sequence

Length: 493 amino acids
MTSSFSRRQFLLGGLVLAGTGAVAACTSDPGPAASAPGPSLRPTPTPTALGEPTVRRTLTARPLSLDIGGIEAKTWGYVSDTGDAAIEATAGDVLQVDITNELPESTSIHWHGIALHNAADGVPGMTQDPIEPGESFSYVFEVPHGGTYFYHSHTGLQLDRGLHAPLIIRDPQDAEDQDVEWTIVLDDWVDGIQGTPDDELDKLTGMGSGDHNGRMGMGGHGQMMHGTPDRVLGGDVGDVMYPHYLINGRIPRAHRTFEARPGDKARLRFINSGGDTIFKVALGGHRMTVTHTDGFPVQPWETESIYLSMGERVDVEVILGDGIFPLTALAVGKDDRAFAVIRTAGGQAPRPDVDFPELSSTGLLLSSLKPADRALLPEGTPDREVSIDLGGQMMPYEWSILTDGQSSSATVQEGQRLRMVMRNRTMMPHPMHIHGHTWALPGSGGLRKDTVLLRHGETMIADLIADNPGEWAFHCHNAYHMETGMVSSLRYE
Esther Ricklefs et al. (2014) β€” Expanding the laccase-toolbox: A laccase from Corynebacterium glutamicum with phenol coupling and cuprous oxidase activity
Journal of Biotechnology  Β· doi:10.1016/j.jbiotec.2014.05.031 β†—  Β· Stability - Incubation
7 measurements ▼
Database ID
UniProt: Q8NLH5 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (7 measurements)

7 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase n-Hexane 50% 80 91 % Incubation: 50 mM potassium phosphate buffer, Assay: 100 mM sodium acetate buffer Incubation: 7.5, Assay: 5 Incubation: Room temperature, Assay: 25Β°C 0.5 mM ABTS ABTS radical cation reaction products β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Isooctane 50% 80 83 % Incubation: 50 mM potassium phosphate buffer, Assay: 100 mM sodium acetate buffer Incubation: 7.5, Assay: 5 Incubation: Room temperature, Assay: 25Β°C 0.5 mM ABTS ABTS radical cation reaction products β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 40% 80 60 % Incubation: 50 mM potassium phosphate buffer, Assay: 100 mM sodium acetate buffer Incubation: 7.5, Assay: 5 Incubation: Room temperature, Assay: 25Β°C 0.5 mM ABTS ABTS radical cation reaction products β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Ethanol 40% 80 94 % Incubation: 50 mM potassium phosphate buffer, Assay: 100 mM sodium acetate buffer Incubation: 7.5, Assay: 5 Incubation: Room temperature, Assay: 25Β°C 0.5 mM ABTS ABTS radical cation reaction products β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Isopropanol 40% 80 80 % Incubation: 50 mM potassium phosphate buffer, Assay: 100 mM sodium acetate buffer Incubation: 7.5, Assay: 5 Incubation: Room temperature, Assay: 25Β°C 0.5 mM ABTS ABTS radical cation reaction products β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetonitrile 40% 80 63 % Incubation: 50 mM potassium phosphate buffer, Assay: 100 mM sodium acetate buffer Incubation: 7.5, Assay: 5 Incubation: Room temperature, Assay: 25Β°C 0.5 mM ABTS ABTS radical cation reaction products β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetone 40% 80 121 % Incubation: 50 mM potassium phosphate buffer, Assay: 100 mM sodium acetate buffer Incubation: 7.5, Assay: 5 Incubation: Room temperature, Assay: 25Β°C 0.5 mM ABTS ABTS radical cation reaction products β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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