Endoglucanase (gene mgcel44) from a mangrove soil metagenomic library
Enzyme Description
Sequence
MGLALDAAGSLYVANCGDYPCGNSNNITIFDAPATQTTATATATPTATTANVTPTPTTTTSTVTATPTATATRTPTPTATSTQVTPTTADISLSVDLSTNRHPISDAIYGIHYVEDEAFAAEIDLPVRRWGGNDTTRYNWKNSMFGNADWYFENEYKPTSADDFIAQNKRTGADSIITLPMSGWMTKDPGQPGNSATYPCSFDTRKYNYTPQALPNGLPSYDTADPKRSHCGSGVTRYENGRAVYFQGNDPTDTSFAIDSSWTSEWIAHLVQTFGAADKNGVRFYGLDNEPDLWHETHRDIFPIALTYDQIRQRAYDYAAVVKAADPAAQILGPVLMGWTYYWHSPRDGQQELWTTRPDRMSHGDVPLVPWYLQQMAEYEAQNGIRLLDYLDLHFYPQNGVDLRDAGDANLQALRLRSTRALWDPTYVDESWIADAGPDGGVVQLIPRMREWVTQNYPGTKLAITEYNWGALDHINGALTQADILGIFGREGLDLATLFDTPYGNGGNFSPQSPAAYAFRLFRNYNGAGAKFGDISVEAVSSDQEKLAVYAAERSSDNALTLLVINKSSSPLTADLSIINRPSSLSTMGQIYRYSAGAPNTIVTAADLSLAGDSTQTTFPASSITLIVVQSSPTDLPHRLYLSALNRH
Zhimao Mai et al. (2014)
β Cloning and characterization of a novel GH44 family endoglucanase from mangrove soil metagenomic library
Biotechnology Letters
Β· doi:10.1007/s10529-014-1531-4 β
Β· Stability - Incubation
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Database ID
UniProt: U5YC86 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (10 measurements)
10 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 15% | 100 | 81 | % | Incubation: ?, Assay: Na2HPO4/citric acid buffer | Incubation: ?, Assay: 6 | Incubation: 25Β°C, Assay: 45Β°C | 1% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Methanol | 15% | 100 | 87 | % | Incubation: ?, Assay: Na2HPO4/citric acid buffer | Incubation: ?, Assay: 6 | Incubation: 25Β°C, Assay: 45Β°C | 1% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Acetonitrile | 15% | 100 | 76 | % | Incubation: ?, Assay: Na2HPO4/citric acid buffer | Incubation: ?, Assay: 6 | Incubation: 25Β°C, Assay: 45Β°C | 1% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Ethanol | 15% | 100 | 78 | % | Incubation: ?, Assay: Na2HPO4/citric acid buffer | Incubation: ?, Assay: 6 | Incubation: 25Β°C, Assay: 45Β°C | 1% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Acetone | 15% | 100 | 88 | % | Incubation: ?, Assay: Na2HPO4/citric acid buffer | Incubation: ?, Assay: 6 | Incubation: 25Β°C, Assay: 45Β°C | 1% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Isopropanol | 15% | 100 | 67 | % | Incubation: ?, Assay: Na2HPO4/citric acid buffer | Incubation: ?, Assay: 6 | Incubation: 25Β°C, Assay: 45Β°C | 1% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Ethyl Acetate | 15% | 100 | 15 | % | Incubation: ?, Assay: Na2HPO4/citric acid buffer | Incubation: ?, Assay: 6 | Incubation: 25Β°C, Assay: 45Β°C | 1% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | 1-Butanol | 15% | 100 | 41 | % | Incubation: ?, Assay: Na2HPO4/citric acid buffer | Incubation: ?, Assay: 6 | Incubation: 25Β°C, Assay: 45Β°C | 1% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Chloroform | 15% | 100 | 39 | % | Incubation: ?, Assay: Na2HPO4/citric acid buffer | Incubation: ?, Assay: 6 | Incubation: 25Β°C, Assay: 45Β°C | 1% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hour at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Toluene | 15% | 100 | 102 | % | Incubation: ?, Assay: Na2HPO4/citric acid buffer | Incubation: ?, Assay: 6 | Incubation: 25Β°C, Assay: 45Β°C | 1% CMC (Carboxymethylcellulose sodium) | Reducing sugars | β | β | Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.