Esterase (gene EstL28) from a swamp sediment metagenomic library
Enzyme Description
Sequence
MGARKSMIQDKTIQLGDVQLHYVEAAGTGPNLILVHGITSALTSYLPLIPALTQDAHVYALDLRGHDQSSHVPGAYHISDYGRDLDSFISTVVGEPVIVAGHSLGALVATWAGANAGTNIRGILLEDPPMYSAQMPRIKAGGAYRYFVHLRQMLDAHAAAGASVDDLAEQLGQSPAGEGQTLLDVMGSEWMRDFTERVHRLDPRVLDAPIEGWLFDGFDPDTDLPRVECPVHLLAGEVSRGGELTTADVQRIATSIPHCTYTVVEAGHGIHDEQPEVYLHALRQMLKAVG
Sohyeon Seo et al. (2013)
β Characterization of a novel cold-active esterase isolated from swamp sediment metagenome
World Journal of Microbiology and Biotechnology
Β· doi:10.1007/s11274-013-1496-9 β
Β· Activity + Stability - Incubation
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Database ID
UniParc: UPI000261797C β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (8 measurements)
8 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Methanol | 5% | 100.0 | 76.4 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8.5, Assay: 8.5 | Incubation: 35Β°C, Assay: 35Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Ethanol | 5% | 100.0 | 66.0 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8.5, Assay: 8.5 | Incubation: 35Β°C, Assay: 35Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Isopropanol | 5% | 100.0 | 83.0 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8.5, Assay: 8.5 | Incubation: 35Β°C, Assay: 35Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | 1-Butanol | 5% | 100.0 | 0.0 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8.5, Assay: 8.5 | Incubation: 35Β°C, Assay: 35Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Acetonitrile | 5% | 100.0 | 26.5 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8.5, Assay: 8.5 | Incubation: 35Β°C, Assay: 35Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 5% | 100.0 | 113.1 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8.5, Assay: 8.5 | Incubation: 35Β°C, Assay: 35Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Acetone | 5% | 100.0 | 53.0 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8.5, Assay: 8.5 | Incubation: 35Β°C, Assay: 35Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Dimethylformamide (DMF) | 5% | 100.0 | 41.3 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8.5, Assay: 8.5 | Incubation: 35Β°C, Assay: 35Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
Visualization : Activity + Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.