N- and C-terminal truncated lipase GD-95 from Geobacillus sp. 95
Enzyme Description
Sequence
AVSRANDAPIVLLHGFTGWGREEMFGFKYWGGVRGDIEQWLNDNGYQAYTLAVGPLSSNWDRACEAYAQLVGGTVDYGAAHAAKHGHARFGRTYPGLLPELKRGGRIHIIAHSQGGQTARMLVSLLENGSQEEREYAKAHNVSLSPLFEGGHRFVLSVTTIATPHDGTTLVNMVDFTDRFFDLQKAVLEAAAVASNAPYTSEIYDFKLDQWGLRREPGESFDHYFERLKRSPVWTSTDTARYDLSVPGAEKLNQWVQASPNTYYLSFSTERTYRGALTGNYYPELGMNAFSAVVCAPFLGSYRNPTLGIDDRWLENDGIVNTVSMNGPKRGSSDRIVPYDGALKKGVWNDMGTYNVDHLEIIGVDPNPSFDIRAFYLR
Renata GudiukaitΖΓ³ et al. (2013)
β Influence of N- and/or C-terminal regions on activity, expression, characteristics and structure of lipase from Geobacillus sp. 95
Extremophiles
Β· doi:10.1007/s00792-013-0605-x β
Β· Stability - Incubation
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Database ID
UniProt: V5JF58 β
Sequence Annotation
Explicit - Provided GenBank Accession Number (28 first and 10 last residues from UniProt sequence absent from the mature protein)
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (7 measurements)
7 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 25% | 100 | 56 | % | Incubation: ?, Assay: 50 mM glycine-NaOH buffer, 10% DMSO (Dimethyl Sulfoxide) | Incubation: ?, Assay: 9 | Incubation: Room temperature, Assay: 55Β°C | 0.25 mM p-Nitrophenyl decanoate | Lauric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Dimethylformamide (DMF) | 25% | 100 | 104 | % | Incubation: ?, Assay: 50 mM glycine-NaOH buffer, 10% DMSO (Dimethyl Sulfoxide) | Incubation: ?, Assay: 9 | Incubation: Room temperature, Assay: 55Β°C | 0.25 mM p-Nitrophenyl decanoate | Lauric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Ethanol | 25% | 100 | 105 | % | Incubation: ?, Assay: 50 mM glycine-NaOH buffer, 10% DMSO (Dimethyl Sulfoxide) | Incubation: ?, Assay: 9 | Incubation: Room temperature, Assay: 55Β°C | 0.25 mM p-Nitrophenyl decanoate | Lauric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Methanol | 25% | 100 | 86 | % | Incubation: ?, Assay: 50 mM glycine-NaOH buffer, 10% DMSO (Dimethyl Sulfoxide) | Incubation: ?, Assay: 9 | Incubation: Room temperature, Assay: 55Β°C | 0.25 mM p-Nitrophenyl decanoate | Lauric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Acetone | 25% | 100 | 131 | % | Incubation: ?, Assay: 50 mM glycine-NaOH buffer, 10% DMSO (Dimethyl Sulfoxide) | Incubation: ?, Assay: 9 | Incubation: Room temperature, Assay: 55Β°C | 0.25 mM p-Nitrophenyl decanoate | Lauric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Isopropanol | 25% | 100 | 110 | % | Incubation: ?, Assay: 50 mM glycine-NaOH buffer, 10% DMSO (Dimethyl Sulfoxide) | Incubation: ?, Assay: 9 | Incubation: Room temperature, Assay: 55Β°C | 0.25 mM p-Nitrophenyl decanoate | Lauric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | 1-Butanol | 25% | 100 | 242 | % | Incubation: ?, Assay: 50 mM glycine-NaOH buffer, 10% DMSO (Dimethyl Sulfoxide) | Incubation: ?, Assay: 9 | Incubation: Room temperature, Assay: 55Β°C | 0.25 mM p-Nitrophenyl decanoate | Lauric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.